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OriGene

NMI Monoclonal Antibody (OTI5H1), TrueMAB™

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite NMI Monoclonal Antibody (OTI5H1), TrueMAB™

  • Antibody Testing Data (2)
NMI Antibody in Western Blot (WB)
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NMI Antibody in Western Blot (WB)
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NMI Antibody (CF810043) in WB

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NMI (RC201543, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-NMI. (1:2. Positive lysates LY417813 (100 µg) and LC417813 (20 µg) can be purchased separately from OriGene. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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NMI Antibody in Western Blot (WB)
NMI Antibody in Western Blot (WB)
NMI Monoclonal Antibody (OTI5H1), TrueMAB™

Product Details

CF810043

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

OTI5H1

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-223 of human NMI produced in E.coli.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: N-myc (and STAT) interactor; N-myc and STAT interactor; N-myc interactor; N-myc-interactor; Nmi

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Gene Aliases: NMI

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UniProt ID: (Human) Q13287

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Entrez Gene ID: (Human) 9111

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Function(s)
transcription cofactor activity protein binding
Process(es)
transcription from RNA polymerase II promoter inflammatory response JAK-STAT cascade negative regulation of interferon-alpha biosynthetic process negative regulation of interferon-beta biosynthetic process negative regulation of innate immune response interferon-gamma-mediated signaling pathway positive regulation of protein K48-linked ubiquitination regulation of nucleic acid-templated transcription
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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