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NMI Monoclonal Antibody (9E8)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite NMI Monoclonal Antibody (9E8)

  • Antibody Testing Data (1)
NMI Antibody in Western Blot (WB)
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NMI Antibody in Western Blot (WB)
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NMI Antibody (H00009111-M04) in WB

NMI monoclonal antibody (M04), clone 9E8. Western Blot analysis of NMI expression in HeLa. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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NMI Antibody in Western Blot (WB)
NMI Monoclonal Antibody (9E8)

Product Details

H00009111-M04

Applications
Tested Dilution
Publications

Western Blot (WB)

1-5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

3 µg/mL
-

ELISA (ELISA)

10 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

9E8

Immunogen

NMI (AAH01268, 1 a.a. approximately 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

Product may be used with Western Blot (Cell lysate).

Immunogen sequence: MEADKDDTQQ ILKEHSPDEF IKDEQNKGLI DEITKKNIQL KKEIQKLETE LQEATKEFQI KEDIPETKMK FLSVETPEND SQLSNISCSF QVSSKVPYEI

Target Information

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: N-myc (and STAT) interactor; N-myc and STAT interactor; N-myc interactor; N-myc-interactor; Nmi

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Gene Aliases: NMI

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UniProt ID: (Human) Q13287

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Entrez Gene ID: (Human) 9111

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Function(s)
transcription cofactor activity protein binding
Process(es)
transcription from RNA polymerase II promoter inflammatory response JAK-STAT cascade negative regulation of interferon-alpha biosynthetic process negative regulation of interferon-beta biosynthetic process negative regulation of innate immune response interferon-gamma-mediated signaling pathway positive regulation of protein K48-linked ubiquitination regulation of nucleic acid-templated transcription
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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