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  • NMI Antibodies

Invitrogen

NMI Monoclonal Antibody (2F3)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite NMI Monoclonal Antibody (2F3)

  • Antibody Testing Data (7)
NMI Antibody in Immunocytochemistry (ICC/IF)
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NMI Antibody in Immunocytochemistry (ICC/IF)
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NMI Antibody (MA5-49239) in ICC/IF

Immunocytochemistry analysis of NMI in SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in NMI Monoclonal antibody (Product # MA5-49239) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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NMI Antibody in Immunocytochemistry (ICC/IF)
NMI Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NMI Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NMI Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NMI Antibody in Western Blot (WB)
NMI Antibody in Flow Cytometry (Flow)
NMI Antibody in Flow Cytometry (Flow)
NMI Monoclonal Antibody (2F3)

Product Details

MA5-49239

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL
-

Immunocytochemistry (ICC/IF)

5 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

2F3

Immunogen

E.coli-derived human NMI recombinant protein (Position: E2-E307).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3074897

Product Specific Information

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Human NMI shares 64% amino acid (aa) sequence identity with mouse NMI.

Positive Control - WB: human placenta tissue, K562 whole cell, Hela whole cell, A431 whole cell, PC-3 whole cell, Caco-2 whole cell, HEK293 whole cell, mouse intestine tissue, rat liver tissue. IHC: human lung cancer tissue, human colon cancer tissue, human placenta tissue. ICC/IF: SiHa cell. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: N-mcy (and STAT) interactor; N-myc (and STAT) interactor; N-myc and STAT interactor; N-myc interactor; N-myc-interactor; Nmi

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Gene Aliases: NMI

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UniProt ID: (Human) Q13287, (Mouse) O35309

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Entrez Gene ID: (Human) 9111, (Mouse) 64685, (Rat) 311021

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Function(s)
transcription cofactor activity protein binding molecular_function
Process(es)
negative regulation of type I interferon production negative regulation of interferon-alpha biosynthetic process negative regulation of interferon-beta biosynthetic process negative regulation of innate immune response positive regulation of protein K48-linked ubiquitination biological_process transcription from RNA polymerase II promoter inflammatory response JAK-STAT cascade interferon-gamma-mediated signaling pathway regulation of nucleic acid-templated transcription
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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