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  • NMI Antibodies

Bethyl Laboratories

NMI Polyclonal Antibody

View all (30) NMI antibodies

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Datasheet
Protocols
Questions & Answers

Cite NMI Polyclonal Antibody

  • Antibody Testing Data (2)
NMI Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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NMI Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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FIGURE: 1 / 2

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NMI Antibody (A300-551A) in IHC (P)

Detection of human NMI by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma (left) and bladder carcinoma (right). Antibody: Affinity purified rabbit anti-NMI (Product # A300-551A) (Lot 1) used at a dilution of 1:1,000 (0.2 µg/mL). Detection: DAB. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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NMI Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NMI Antibody in Western Blot (WB)
NMI Polyclonal Antibody

Product Details

A300-551A

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000-1:10,000
-

Immunohistochemistry (IHC)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000
-

Immunoprecipitation (IP)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 250 and the C-terminus (residue 307) of human N-myc and STAT interactor.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.20 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.0 to 8.0, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: N-myc (and STAT) interactor; N-myc and STAT interactor; N-myc interactor; N-myc-interactor; Nmi

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Gene Aliases: NMI

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UniProt ID: (Human) Q13287

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Entrez Gene ID: (Human) 9111

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Function(s)
transcription cofactor activity protein binding
Process(es)
transcription from RNA polymerase II promoter inflammatory response JAK-STAT cascade negative regulation of interferon-alpha biosynthetic process negative regulation of interferon-beta biosynthetic process negative regulation of innate immune response interferon-gamma-mediated signaling pathway positive regulation of protein K48-linked ubiquitination regulation of nucleic acid-templated transcription
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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