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OriGene

NME1 Monoclonal Antibody (OTI7F2), TrueMAB™

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Cite NME1 Monoclonal Antibody (OTI7F2), TrueMAB™

  • Antibody Testing Data (9)
NME1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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NME1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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NME1 Antibody (CF801264) in IHC (P)

Immunohistochemical staining of paraffin-embedded human lymphoma tissue using anti-NME1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801264) {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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NME1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NME1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NME1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NME1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NME1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NME1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NME1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NME1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NME1 Antibody in Western Blot (WB)
NME1 Monoclonal Antibody (OTI7F2), TrueMAB™

Product Details

CF801264

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:150
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI7F2

Immunogen

Full length human recombinant protein of human NME1 produced in E.coli.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated
  • Biotin 
  • Request custom conjugation

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

NME1 was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in the gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: GAAD; Granzyme A activated DNase (GAAD); Granzyme A-activated DNase; GZMA activated DNase; included; Metastasis inhibition factor nm23; NDK A; NDP kinase A; NM23-H1; NM23H1B; NME1-NME2 spliced read-through transcript; non-metastatic cells 1, protein (NM23A) expressed in; Nucleoside diphosphate kinase A; Tumor metastatic process-associated protein

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Gene Aliases: AWD; GAAD; NB; NBS; NDKA; NDPK-A; NDPKA; NM23; NM23-H1; NME1

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UniProt ID: (Human) P15531

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Entrez Gene ID: (Human) 4830

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Function(s)
magnesium ion binding RNA polymerase II regulatory region sequence-specific DNA binding single-stranded DNA binding deoxyribonuclease activity nucleoside diphosphate kinase activity protein binding ATP binding GTP binding intermediate filament binding protein kinase binding identical protein binding gamma-tubulin binding ribosomal small subunit binding poly(A) RNA binding
Process(es)
negative regulation of myeloid leukocyte differentiation purine nucleotide metabolic process nucleoside diphosphate phosphorylation GTP biosynthetic process pyrimidine nucleotide metabolic process UTP biosynthetic process CTP biosynthetic process DNA metabolic process endocytosis lactation negative regulation of cell proliferation nucleoside triphosphate biosynthetic process negative regulation of gene expression positive regulation of neuron projection development response to amine nucleobase-containing small molecule interconversion hippocampus development response to testosterone cellular response to drug regulation of apoptotic process positive regulation of DNA binding positive regulation of epithelial cell proliferation response to cAMP cellular response to glucose stimulus cellular response to fatty acid
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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