Invitrogen

NME1 Monoclonal Antibody (13C1)

Name: NME1 Monoclonal Antibody (13C1)
Brand: Invitrogen
SKU: MA5-34938

View all (121) NME1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (6)

NME1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 6

NME1 Antibody (MA5-34938) in IHC (P)

Immunohistochemistry analysis of NM23 in paraffin-embedded human breast carcinoma tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with NM23 monoclonal antibody (Product # MA5-34938) at a dilution of 1:50 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with D... View More

Product Image Gallery

Immunogen image

Immunogen Image NME1 Monoclonal Antibody (13C1)

Product Details

MA5-34938

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Immunocytochemistry (ICC/IF)

1:100

Flow Cytometry (Flow)

1:50-1:100

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

13C1

Immunogen

Synthetic peptide within Human NM23 aa 1-50

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 50% glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2848844

Product Specific Information

Positive Control: K562 cell, A549 cell, HepG2 cell, 293 cell, human liver carcinoma tissue, human thyroid tissue, human skin tissue, human breast carcinoma tissue.

Target Information

NME1 was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in the gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: GAAD; Granzyme A activated DNase (GAAD); Granzyme A-activated DNase; GZMA activated DNase; included; Metastasis inhibition factor nm23; NDK A; NDP kinase A; NM23-H1; NM23H1B; NME1-NME2 spliced read-through transcript; non-metastatic cells 1, protein (NM23A) expressed in; Nucleoside diphosphate kinase A; Tumor metastatic process-associated protein

View more View less

Gene Aliases: AWD; GAAD; NB; NBS; NDKA; NDPK-A; NDPKA; NM23; NM23-H1; NME1

View more View less

UniProt ID: (Human) P15531

View more View less

Entrez Gene ID: (Human) 4830

View more View less

Function(s)

magnesium ion binding RNA polymerase II regulatory region sequence-specific DNA binding single-stranded DNA binding deoxyribonuclease activity nucleoside diphosphate kinase activity protein binding ATP binding GTP binding intermediate filament binding protein kinase binding identical protein binding gamma-tubulin binding ribosomal small subunit binding poly(A) RNA binding

Process(es)

negative regulation of myeloid leukocyte differentiation purine nucleotide metabolic process nucleoside diphosphate phosphorylation GTP biosynthetic process pyrimidine nucleotide metabolic process UTP biosynthetic process CTP biosynthetic process DNA metabolic process endocytosis lactation negative regulation of cell proliferation nucleoside triphosphate biosynthetic process negative regulation of gene expression positive regulation of neuron projection development response to amine nucleobase-containing small molecule interconversion hippocampus development response to testosterone cellular response to drug regulation of apoptotic process positive regulation of DNA binding positive regulation of epithelial cell proliferation response to cAMP cellular response to glucose stimulus cellular response to fatty acid

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

NME1 Monoclonal Antibody (13C1)

NME1 Antibody (MA5-34938) in IHC (P)

Product Details

MA5-34938

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!