Invitrogen

NME1 Recombinant Rabbit Monoclonal Antibody (084)

Name: NME1 Recombinant Rabbit Monoclonal Antibody (084)
Brand: Invitrogen
SKU: MA5-29447

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Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Advanced Verification (1)

NME1 Antibody in Immunocytochemistry (ICC/IF)

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NME1 Antibody (MA5-29447) in ICC/IF

Immunofluorescence analysis NME1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NME1 Monoclonal Antibody (Product # MA5-29447) at 1:100 in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A28175) at a dilution of 1:2000 f... View More

NME1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

NME1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with NME1 siRNA and reduction of signal was observed in Western Blot using NME1 Recombinant Rabbit Monoclonal Antibody (Product # MA5-29447). {KD}

Immunogen image

Immunogen Image NME1 Recombinant Rabbit Monoclonal Antibody (084)

Product Details

MA5-29447

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

084

Immunogen

Recombinant Human NME1/NDKA protein (Ala2-Glu152)

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2785309

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

This antibody has specificity for Human NME1/NDKA.

Target Information

NME1 was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in the gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: GAAD; Granzyme A activated DNase (GAAD); Granzyme A-activated DNase; GZMA activated DNase; included; Metastasis inhibition factor nm23; NDK A; NDP kinase A; NM23-H1; NM23H1B; NME1-NME2 spliced read-through transcript; non-metastatic cells 1, protein (NM23A) expressed in; Nucleoside diphosphate kinase A; Tumor metastatic process-associated protein

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Gene Aliases: AWD; GAAD; NB; NBS; NDKA; NDPK-A; NDPKA; NM23; NM23-H1; NME1

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UniProt ID: (Human) P15531

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Entrez Gene ID: (Human) 4830

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Function(s)

magnesium ion binding RNA polymerase II regulatory region sequence-specific DNA binding single-stranded DNA binding deoxyribonuclease activity nucleoside diphosphate kinase activity protein binding ATP binding GTP binding intermediate filament binding protein kinase binding identical protein binding gamma-tubulin binding ribosomal small subunit binding poly(A) RNA binding

Process(es)

negative regulation of myeloid leukocyte differentiation purine nucleotide metabolic process nucleoside diphosphate phosphorylation GTP biosynthetic process pyrimidine nucleotide metabolic process UTP biosynthetic process CTP biosynthetic process DNA metabolic process endocytosis lactation negative regulation of cell proliferation nucleoside triphosphate biosynthetic process negative regulation of gene expression positive regulation of neuron projection development response to amine nucleobase-containing small molecule interconversion hippocampus development response to testosterone cellular response to drug regulation of apoptotic process positive regulation of DNA binding positive regulation of epithelial cell proliferation response to cAMP cellular response to glucose stimulus cellular response to fatty acid

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NME1 Recombinant Rabbit Monoclonal Antibody (084)

NME1 Antibody (MA5-29447) in ICC/IF

Product Details

MA5-29447

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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