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Invitrogen

NG2 Recombinant Rabbit Monoclonal Antibody (6C9L7)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite NG2 Recombinant Rabbit Monoclonal Antibody (6C9L7)

  • Antibody Testing Data (1)
NG2 Antibody in Western Blot (WB)
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NG2 Antibody in Western Blot (WB)
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NG2 Antibody (MA5-55300) in WB

Western blot analysis of lysates from A375 cells, using NG2 Recombinant Monoclonal Antibody (Product # MA5-55300) at 1:1,000 dilution followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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NG2 Antibody in Western Blot (WB)
NG2 Recombinant Rabbit Monoclonal Antibody (6C9L7)

Product Details

MA5-55300

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

6C9L7

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1340-1563 of human NG2/CSPG4.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.05% ProClin 300

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

RRID

AB_3667542

Product Specific Information

Immunogen Sequence: LGAPLEGVLV ELEVLPAAIP LEAQNFSVPE GGSLTLAPPL LRVSGPYFPT LLGLSLQVLE PPQHGALQKE DGPQARTLSA FSWRMVEEQL IRYVHDGSET LTDSFVLMAN ASEMDRQSHP VAFTVTVLPV NDQPPILTTN TGLQMWEGAT APIPAEALRS TDGDSGSEDL VYTIEQPSNG RVVLRGAPGT EVRSFTQAQL DGGLVLFSHR GTLDGGFRFR LSDG

Target Information

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Chondroitin sulfate proteoglycan 4; chondroitin sulfate proteoglycan 4 (melanoma-associated); Chondroitin sulfate proteoglycan NG2; HMW MAA; MEL CSPG; Melanoma chondroitin sulfate proteoglycan; Melanoma-associated chondroitin sulfate proteoglycan

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Gene Aliases: CSPG4; HMW-MAA; MCSP; MCSPG; MEL-CSPG; MSK16; NG2

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UniProt ID: (Human) Q6UVK1

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Entrez Gene ID: (Human) 1464

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Function(s)
signal transducer activity protein kinase binding
Process(es)
activation of MAPK activity angiogenesis transmembrane receptor protein tyrosine kinase signaling pathway cell proliferation glial cell migration cell migration glycosaminoglycan metabolic process chondroitin sulfate biosynthetic process chondroitin sulfate catabolic process dermatan sulfate biosynthetic process intracellular signal transduction tissue remodeling positive regulation of peptidyl-tyrosine phosphorylation
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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