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  • Primary Antibodies ›
  • NF-H Antibodies
Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

Invitrogen

NF-H Recombinant Superclonal™ Antibody (4HCLC)

Advanced Verification
1 Published Figure
1 Reference
View all (65) NF-H antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite NF-H Recombinant Superclonal™ Antibody (4HCLC)

  • Antibody Testing Data (3)
  • Published Figures (1)
  • Advanced Verification (2)
NF-H Antibody in Immunocytochemistry (ICC/IF)
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NF-H Antibody in Immunocytochemistry (ICC/IF)
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NF-H Antibody (711025) in ICC/IF

For immunofluorescence analysis, retinoic acid (10 uM, 3days) treated SH-SY5Y cells were fixed and permeabilized for detection of Neurofilament-H using Neurofilament-H Recombinant Rabbit Superclonal™ Antibody (Product # 711025, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Neurofilament-H protein (green), Panel b) is stained for nuclei (blue) using Slo... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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NF-H Antibody in Immunocytochemistry (ICC/IF)
NF-H Antibody in Immunohistochemistry (Frozen) (IHC (F))
NF-H Antibody in Western Blot (WB)
NF-H Antibody in Immunocytochemistry (ICC/IF)
NF-H Antibody
NF-H Antibody
NF-H Recombinant Superclonal™ Antibody (4HCLC)

Product Details

711025

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent
-

Immunocytochemistry (ICC/IF)

2 µg/mL
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Clone

4HCLC

Immunogen

Peptide corresponding to Human NEFH (aa 631-643)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2609477

Product Specific Information

This antibody is predicted to react with Cat, Dog, Monkey and Bovine.

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

Target Information

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H has an important role in mature axons and is involved in amyotrophic lateral sclerosis (ALS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 200 kDa neurofilament protein; NEF3; neurofilament; neurofilament 200kDa; Neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; NF 200 kD; NF-H; NHC

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Gene Aliases: CMT2CC; KIAA0845; mKIAA0845; NEFH; NF-H; NF200; NFH

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UniProt ID: (Human) P12036, (Mouse) P19246

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Entrez Gene ID: (Human) 4744, (Rat) 24587, (Mouse) 380684

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Function(s)
structural molecule activity structural constituent of cytoskeleton microtubule binding toxic substance binding kinesin binding protein kinase binding protein binding, bridging dynein binding protein heterodimerization activity nutrient reservoir activity
Process(es)
microtubule cytoskeleton organization ovarian follicle atresia axonogenesis spinal cord development hippocampus development cerebral cortex development cell projection assembly axon regeneration neurofilament bundle assembly cellular response to oxidative stress response to cocaine peripheral nervous system neuron axonogenesis axon development cellular response to estradiol stimulus regulation of organelle transport along microtubule response to sodium arsenite response to acrylamide brain development embryo development neuron projection development intermediate filament cytoskeleton organization intermediate filament bundle assembly neurofilament cytoskeleton organization
It has to be done as per old AB suggested Products section.
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