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OriGene

NAGA Monoclonal Antibody (OTI7F1), TrueMAB™

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Datasheet
Protocols
Questions & Answers

Cite NAGA Monoclonal Antibody (OTI7F1), TrueMAB™

  • Antibody Testing Data (1)
NAGA Antibody in Western Blot (WB)
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NAGA Antibody in Western Blot (WB)
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NAGA Antibody (CF811277) in WB

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAGA (RC200707, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-NAGA. (1. Positive lysates LY400100 (100 µg) and LC400100 (20 µg) can be purchased separately from OriGene. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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NAGA Antibody in Western Blot (WB)
NAGA Monoclonal Antibody (OTI7F1), TrueMAB™

Product Details

CF811277

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

OTI7F1

Immunogen

Full length human recombinant protein of human NAGA produced in HEK293T cell.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

NAGA (N-acetylgalactosaminidase, alpha), also known as alpha-galactosidase B or GALB, is a 411 lysosomal protein belonging to the glycosyl hydrolase 27 family that may exist as a homodimer and plays a critical role in glycolipid breakdown. NAGA encodes alpha-N-acetylgalactosaminidase, a lysosomal enzyme, which cleaves alpha-N-acetylgalactosaminyl groups from glycoconjugates. Mapping to human chromosome 22q13. 2, NAGA defects are the cause of an autosomal recessive disorder with three phenotypes, known as Schindler disease (types I, II and III) or NAGA deficiency (types I, II and III). Characterized by neurologic manifestations that range in severity, Schindler disease type I is the most severe form, followed by type III, which may have mild-to-moderate effects. Schindler disease type II, also known as Kanzaki disease, is characterized by mild intellectual impairment and angiokeratoma corporis diffusum.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Acetylgalactosaminidase, alpha-N- (alpha-galactosidase B); Alpha-galactosidase B; Alpha-N-acetylgalactosaminidase; EC 3.2.1; EC 3.2.1.49; galactosidase-alphaB; galactosidasealphaB; N-acetylgalactosaminidase, alpha-

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Gene Aliases: D22S674; GALB; NAGA

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UniProt ID: (Human) P17050

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Entrez Gene ID: (Human) 4668

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Function(s)
alpha-galactosidase activity alpha-N-acetylgalactosaminidase activity protein homodimerization activity
Process(es)
carbohydrate catabolic process glycoside catabolic process glycolipid catabolic process
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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