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  • Mouse anti-Rat IgG2b Secondary Antibodies

Invitrogen

Mouse anti-Rat IgG2b Secondary Antibody, Biotin

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View all (12) Mouse anti-Rat IgG2b antibodies

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Datasheet
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Cite Mouse anti-Rat IgG2b Secondary Antibody, Biotin

  • Antibody Testing Data (1)
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Rat IgG2b Secondary Antibody in Western Blot (WB)
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Rat IgG2b Secondary Antibody in Western Blot (WB)
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Rat IgG2b Secondary Antibody (SA1-25268) in WB

Western blot was performed using Mouse anti-Rat IgG2b Secondary Antibody, Biotin (Product # SA1-25268) and ~55 kDa bandcorresponding to heavy Chain was observed in Rat IgG and Rat IgG2b but not in Rat IgG2a, Rabbit IgG, Goat IgG, Chicken IgY, Mouse IgG, Mouse IgM, Human IgG and Human IgM. Purified protein (100 ng) of Rat IgG (Lane 1), Rat IgG2a (lane 2), Rat IgG2b (Lane 3), Rabbit IgG (Lane 4), Goat IgG (Lane 5), Chicken IgY (Lane 6), Mouse IgG (Lane 7), Mouse IgM (Lane 8), Human IgG (Lane 9) and Human IgM (Lane 10) (Fig a) wereelectrophor... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Rat IgG2b Secondary Antibody in Western Blot (WB)
Rat IgG2b Secondary Antibody

Product Details

SA1-25268

Applications
Tested Dilution

Western Blot (WB)

1:2000

ELISA (ELISA)

0.5 µg/mL
Product Specifications

Species Reactivity

Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Secondary Antibody

Clone

MARG2b-8

Immunogen

IR863 rat IgG2b myeloma protein.

Conjugate

Biotin Biotin Biotin
  • eFluor 570
  • eFluor 660
  • FITC
  • PE 
  • Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_794598

Target

IgG2b

Antibody Form

Whole Antibody

Product Specific Information

Store as concentrated solution. Centrifuge briefly prior to opening vial.

Target Information

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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