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  • Mouse anti-Rat IgG2b (Heavy chain) Secondary Antibodies

Invitrogen

Mouse anti-Rat IgG2b (Heavy chain) Secondary Antibody

View all (3) Mouse anti-Rat IgG2b (Heavy chain) antibodies

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Cite Mouse anti-Rat IgG2b (Heavy chain) Secondary Antibody

  • Antibody Testing Data (2)
Rat IgG2b (Heavy chain) Secondary Antibody in Western Blot (WB)
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Rat IgG2b (Heavy chain) Secondary Antibody in Western Blot (WB)
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Rat IgG2b (Heavy chain) Secondary Antibody (SA1-25266) in WB

Fluorescent western blot was performed usingMouse anti-Rat IgG2b (Heavy chain) Secondary Antibody(Product # SA1-25266). Whole cell extracts of HeLa (Lane 1, 2, 3, 4, 5) and HEK-293 (Lane 6, 7, 8, 9, 10) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP03222BOX). Resolved proteins were transferred onto anitrocellulose membrane(Product # IB23001) byiBlot® 2 Dry BlottingSystem (Produc... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Rat IgG2b (Heavy chain) Secondary Antibody in Western Blot (WB)
Rat IgG2b (Heavy chain) Secondary Antibody in Western Blot (WB)

Product Details

SA1-25266

Applications
Tested Dilution

Western Blot (WB)

1:10,000

ELISA (ELISA)

5 µg/mL
Product Specifications

Species Reactivity

Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Secondary Antibody

Clone

MARG2b-3

Immunogen

Rat IgG2b.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_794596

Target

IgG2b (Heavy chain)

Antibody Form

Whole Antibody

Product Specific Information

SA1-25266 detects rat IgG2b, Heavy Chain.

Target Information

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

View product citations for antibody SA1-25266 on CiteAb

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