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  • Isotype Controls ›
  • Mouse IgG2a Isotype Controls

Invitrogen

Mouse IgG2a Isotype Control (MOPC-173), DyLight™ 488

View all Mouse IgG2a Isotype Controls

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Cite Mouse IgG2a Isotype Control (MOPC-173), DyLight™ 488

  • Testing Data (3)
Mouse IgG2a Isotype Control in Immunocytochemistry (ICC/IF)
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Mouse IgG2a Isotype Control in Immunocytochemistry (ICC/IF)
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FIGURE: 1 / 3

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Mouse IgG2a Isotype Control (MA1-193-D488) in ICC/IF

Imunofluorescent analysis of Lin28 (green) in Human episomal iPSC cells. The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 30 minutes at room temperature. Cells were stained with Dylight 488-conjugated Lin28 monoclonal antibody (right, Product # MA1-016-D488) or Dylight 488-conjugated Mouse IgG... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Mouse IgG2a Isotype Control in Immunocytochemistry (ICC/IF)
Mouse IgG2a Isotype Control in Flow Cytometry (Flow)
Mouse IgG2a Isotype Control in Flow Cytometry (Flow)

Product Details

MA1-193-D488

Applications
Tested Dilution

Western Blot (WB)

Assay-dependent

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

Assay-dependent

Flow Cytometry (Flow)

Assay-dependent

Control (Ctrl)

Assay-dependent
Product Specifications

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Isotype Control

Clone

MOPC-173

Immunogen

The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice

Conjugate

DyLight™ 488 DyLight™ 488 DyLight™ 488
  • DyLight 650
  • Request custom conjugation

Excitation/Emission Max

492/519 nm View spectra spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Size-exclusion chromatography

Storage buffer

PBS with 0.2% BSA

Contains

15mM sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536960

Product Specific Information

This antibody reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.

MA1-193-D488 can be used as a negative control in any immunoassay where a DyLight 488-conjugated primary antibody of the mouse IgG2a isotype is used. DyLight 488 has an excitation/emission of 493/518nm.

Target Information

Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2a is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2a is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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