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  • Primary Antibodies ›
  • Mitofilin Antibodies

Invitrogen

Mitofilin Polyclonal Antibody

Advanced Verification
View all (20) Mitofilin antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Cite Mitofilin Polyclonal Antibody

  • Antibody Testing Data (6)
  • Advanced Verification (1)
Mitofilin Antibody in Immunocytochemistry (ICC/IF)
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Mitofilin Antibody in Immunocytochemistry (ICC/IF)
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Mitofilin Antibody (PA5-96203) in ICC/IF

Immunofluorescence analysis of Mitofilin was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Mitofilin Polyclonal Antibody (Product # PA5-96203) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Mitofilin Antibody in Immunocytochemistry (ICC/IF)
Mitofilin Antibody in Immunocytochemistry (ICC/IF)
Mitofilin Antibody in Western Blot (WB)
Mitofilin Antibody in Western Blot (WB)
Mitofilin Antibody in Western Blot (WB)
Mitofilin Antibody in Western Blot (WB)
Mitofilin Antibody
Mitofilin Polyclonal Antibody

Product Details

PA5-96203

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 120-270 of human IMMT (NP_0068302)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2808005

Product Specific Information

Immunogen sequence: ESKQPASQLQ KQKGDTPASA TAPTEAAQII SAAGDTLSVP APAVQPEESL KTDHPEIGEG KPTPALSEEA SSSSIRERPP EEVAARLAQQ EKQEQVKIES LAKSLEDALR QTASVTLQAI AAQNAAVQAV NAHSNILKAA MDNSEIAGEK K; Positive Samples: MCF7, B cells, HeLa, HepG2, SH-SY5Y, HL-60, Mouse kidney; Cellular Location: Mitochondrion, Mitochondrion inner membrane, Single-pass membrane protein

Target Information

Mitofilin is a transmembrane protein of the inner mitochondrial membrane and it is associated with a large multimeric protein complex of about 1200 kDa. Mitofilin has critical functions in mitochondrial morphology and mitochondrial fusion and fission, specifically in the formation of tubular cristae and cristae junctions. Abnormal mitochondrial morphology has been implicated in many human diseases such as myopathy, cardiomyopathy, rhabdomyosarcoma and Whartin's tumor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cell proliferation-inducing gene 4/52 protein; cell proliferation-inducing protein 52; IMMT antibody; MICOS complex subunit MIC60; mitochondrial inner membrane organizing system 2; Mitochondrial inner membrane protein; Mitofilin; motor protein; p87/89; p87/89 antibody; proliferation-inducing gene 4

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Gene Aliases: 1700082C19Rik; D830041H16Rik; HMP; IMMT; MIC60; MINOS2; P87; P87/89; P89; PIG4; PIG52

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UniProt ID: (Human) Q16891, (Mouse) Q8CAQ8

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Entrez Gene ID: (Human) 10989, (Mouse) 76614

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Function(s)
protein binding poly(A) RNA binding
Process(es)
biological_process cristae formation mitochondrial calcium ion homeostasis
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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