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  • Primary Antibodies ›
  • MYL9 Antibodies

Invitrogen

MYL9 Polyclonal Antibody

Advanced Verification
3 Published Figures
3 References
View all (22) MYL9 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite MYL9 Polyclonal Antibody

  • Antibody Testing Data (1)
  • Published Figures (3)
  • Advanced Verification (1)
MYL9 Antibody in Western Blot (WB)
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MYL9 Antibody in Western Blot (WB)
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MYL9 Antibody (PA5-17624) in WB

Western blot was performed using Anti-MYL9 Polyclonal Antibody (Product # PA5-17624) and a 20 kDa band corresponding to Myosin regulatory light polypeptide 9 was observed across cell lines and tissue extract tested and not in Daudi, Mouse Skeletal Muscle and Mouse Liver which is reported to be negative. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Caco-2 (Lane 2), U-2 OS (Lane 3), Jurkat (Lane 4), MDA-MB-231 (Lane 5), C2C12 (Lane 6), Daudi (Lane 7) and tissue extracts Mouse Skeletal Muscle (Lane 8), Mouse Liver (Lane 9) and Mouse U... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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MYL9 Antibody in Western Blot (WB)
MYL9 Antibody in Western Blot (WB)
MYL9 Antibody in Western Blot (WB)
MYL9 Antibody in Western Blot (WB)
MYL9 Antibody
MYL9 Polyclonal Antibody

Product Details

PA5-17624

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to amino-terminal residues of human Myosin Light Chain 2 (smooth muscle isoform)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

19 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10985405

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with the cardiac isoform of Myosin Light Chain 2, or Myosin Essential Light Chain.

Target Information

Myosin, a structural component of muscle, consists of two heavy chains and four light chains. Myosin light chain may regulate muscle contraction by modulating the ATPase activity of myosin heads. It also binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 20 kDa myosin light chain; LC20; MGC3505; MLC-2C; myosin light chain, regulatory C; myosin regulatory light chain; myosin regulatory light chain 1; Myosin regulatory light chain 2, smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1; Myosin regulatory light polypeptide 9; Myosin RLC; myosin, light chain 9, regulatory; myosin, light polypeptide 9, regulatory

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Gene Aliases: AI327049; LC20; MLC-2C; MLC2; MLC20; MRLC1; MYL9; Mylc2c; MYRL2; RLC-C

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UniProt ID: (Human) P24844, (Rat) Q64122, (Mouse) Q9CQ19

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Entrez Gene ID: (Human) 10398, (Rat) 296313, (Mouse) 98932

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Function(s)
calcium ion binding structural constituent of muscle myosin heavy chain binding metal ion binding
Process(es)
platelet aggregation muscle contraction regulation of muscle contraction
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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