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  • MYH10 Antibodies

Invitrogen

MYH10 Polyclonal Antibody

View all (14) MYH10 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite MYH10 Polyclonal Antibody

  • Antibody Testing Data (1)
MYH10 Antibody in Western Blot (WB)
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MYH10 Antibody in Western Blot (WB)
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MYH10 Antibody (PA5-88304) in WB

Western blot analysis of MYH10 in extracts of various cell lines. Samples were incubated with MYH10 Polyclonal antibody (Product # PA5-88304) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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MYH10 Antibody in Western Blot (WB)
MYH10 Polyclonal Antibody

Product Details

PA5-88304

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1908-2007 of human MYH10 (NP_0012429411)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.41 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804815

Product Specific Information

Immunogen sequence: ARMKQLKRQL EEAEEEATRA NASRRKLQRE LDDATEANEG LSREVSTLKN RLRRGGPISF SSSRSGRRQL HLEGASLELS DDDTESKTSD VNETQPPQSE; Positive Samples: HepG2, NIH/3T3, HT-29, Mouse brain, Mouse kidney, Rat brain; Cellular Location: Cell projection, lamellipodium

Target Information

MYH10 encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cellular myosin heavy chain, type B; flectin; MCH-B(B2); Myosin heavy chain 10; myosin heavy chain 10, non-muscle; Myosin heavy chain, non-muscle IIb; myosin heavy chain, nonmuscle type B; myosin heavy chain, nonmuscular, type B; myosin IIB; myosin, heavy polypeptide 10, non-muscle; Myosin-10; NMMHC II-b; NMMHC IIB; NMMHC-B; NMMHC-IIB; Non-muscle myosin heavy chain B; Non-muscle myosin heavy chain IIb; nonmuscle myosin; nonmuscle myosin heavy chain II-B; nonmuscle myosin heavy chain IIB; nonmuscle myosin heavy chain-B; nonmuscle myosin II heavy chain-B; OTTHUMP00000165799

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Gene Aliases: 5730504C04Rik; 9330167F11Rik; Fltn; MCH-B; mKIAA3005; MYH10; Myhn-2; Myhn2; NMHC II-B; NMHC-B; NMHCII-B; NMMHC II-b; NMMHC-B; NMMHC-IIB; NMMHCB; SMemb

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UniProt ID: (Human) P35580, (Mouse) Q61879, (Rat) Q9JLT0

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Entrez Gene ID: (Human) 4628, (Mouse) 77579, (Rat) 79433

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Function(s)
microfilament motor activity actin binding protein binding calmodulin binding ATP binding actin-dependent ATPase activity ADP binding actin filament binding nucleotide binding motor activity
Process(es)
mitotic cytokinesis in utero embryonic development neuron migration plasma membrane repair exocytosis substrate-dependent cell migration, cell extension nuclear migration cell adhesion axonogenesis axon guidance brain development adult heart development metabolic process cell proliferation regulation of cell shape fourth ventricle development lateral ventricle development third ventricle development cerebellar Purkinje cell layer development actin cytoskeleton organization actin filament-based movement myofibril assembly actomyosin structure organization neuron projection development neuromuscular process controlling balance cardiac myofibril assembly ventricular cardiac muscle cell development retina development in camera-type eye cardiac septum development aorta development coronary vasculature development
It has to be done as per old AB suggested Products section.
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