Invitrogen

MST1 (STK4) Polyclonal Antibody

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Datasheet
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Datasheet
Protocols
Questions & Answers
MST1 (STK4) Polyclonal Antibody

Product Details

PA5-96907

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of
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Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 303-487 of human MST1/STK4 (NP_0062731)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2808709

Product Specific Information

Immunogen sequence: KRQESQQREV DQDDEENSEE DEMDSGTMVR AVGDEMGTVR VASTMTDGAN TMIEHDDTLP SQLGTMVINA EDEEEEGTMK RRDETMQPAK PSFLEYFEQK EKENQINSFG KSVPGPLKNS SDWKIPQDGD YEFLKSWTVE DLQKRLLALD PMMEQEIEEI RQKYQSKRQP ILDAIEAKKR RQQNF; Positive Samples: U-87MG, HepG2, HeLa, MCF-7, Mouse brain, Mouse lung, Mouse spleen, Rat liver; Cellular Location: Cytoplasm, Nucleus

Target Information

STK4 (MST1) is a serine/threonine kinase which may be involved in apoptosis. It is possible that this protein induces the chromatin condensation observed in this process. STK4 is a stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. It is a key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EC 2.7.11.1; kinase MST1; kinase responsive to stress 2; Mammalian STE20-like protein kinase 1; mammalian sterile 20-like 1; MST-1; Serine/threonine-protein kinase 4; Serine/threonine-protein kinase Krs-2; STE20-like kinase MST1; sterile 20-like kinase 1; TIIAC; Yeast Sps1/Ste20-related kinase 3

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Gene Aliases: AI447339; AU020804; Kas-2; KRS2; MST1; STK4; YSK3

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UniProt ID: (Human) Q13043, (Mouse) Q9JI11

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Entrez Gene ID: (Human) 6789, (Rat) 311622, (Mouse) 58231

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Function(s)
magnesium ion binding protein kinase activity protein serine/threonine kinase activity receptor signaling protein serine/threonine kinase activity protein binding ATP binding transcription factor binding identical protein binding protein homodimerization activity protein serine/threonine kinase activator activity protein dimerization activity nucleotide binding MAP kinase kinase kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups metal ion binding
Process(es)
cell morphogenesis patterning of blood vessels neural tube formation endocardium development protein phosphorylation apoptotic process central nervous system development negative regulation of cell proliferation peptidyl-serine phosphorylation signal transduction by protein phosphorylation keratinocyte differentiation positive regulation of protein binding hippo signaling intracellular signal transduction positive regulation of apoptotic process positive regulation of fat cell differentiation negative regulation of organ growth protein autophosphorylation primitive hemopoiesis cell differentiation involved in embryonic placenta development regulation of cell differentiation involved in embryonic placenta development negative regulation of canonical Wnt signaling pathway hepatocyte apoptotic process positive regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of protein phosphorylation signal transduction positive regulation of peptidyl-serine phosphorylation protein stabilization positive regulation of protein serine/threonine kinase activity MAPK cascade activation of MAPKK activity regulation of mitotic cell cycle phosphorylation stress-activated protein kinase signaling cascade
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