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  • Primary Antibodies ›
  • MRLC2 Antibodies

Invitrogen

MRLC2 Polyclonal Antibody

Advanced Verification
View all (5) MRLC2 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite MRLC2 Polyclonal Antibody

  • Antibody Testing Data (4)
  • Advanced Verification (1)
MRLC2 Antibody in Western Blot (WB)
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MRLC2 Antibody in Western Blot (WB)
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MRLC2 Antibody (PA5-78618) in WB

Western blot was performed using Anti-MRLC2 Rabbit Polyclonal Antibody (Product # PA5-78618) and a 20 kDa band corresponding to MRLC2 was observed in all the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of Jurkat (Lane1), Raji (Lane2), K-562 (Lane 3), HT-29 (Lane 4), RD (Lane 5), A549 (Lane 6), NIH-3T3 (Lane 7), HeLa (Lane 8), tissue extracts (30 µg lysate) of Mouse Heart (Lane 9) and Mouse Skeletal Muscle (Lane 10) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0322BOX). Resolved proteins we... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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MRLC2 Antibody in Western Blot (WB)
MRLC2 Antibody in Western Blot (WB)
MRLC2 Antibody in Western Blot (WB)
MRLC2 Antibody in Western Blot (WB)
MRLC2 Antibody

Product Details

PA5-78618

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:10,000
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human MRLC2. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2736164

Product Specific Information

Positive Control: K562, Jurkat, Raji, NCI-H929

Predicted Reactivity: Mouse (100%), Rat (100%), Xenopus laevis (95%), Rhesus Monkey (100%), Bovine (98%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

MRLC2 is involved in the the activity of nonmuscle myosin II is regulated by phosphorylation of a regulatory light chain. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: MLC-2; MLC-2A; MLC20; MLCB; MRLC3; myosin light chain, regulatory B; Myosin regulatory light chain 12B; myosin regulatory light chain 2; Myosin regulatory light chain 2-B, smooth muscle isoform; Myosin regulatory light chain 20 kDa; Myosin regulatory light chain MRLC2; myosin, light chain 12B, regulatory; RLC; SHUJUN-1

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Gene Aliases: 1500001M02Rik; C77744; MLC-B; MRLC2; MYL12B; MYLC2B; RLC-B

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UniProt ID: (Human) O14950, (Mouse) Q3THE2

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Entrez Gene ID: (Human) 103910, (Mouse) 67938

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Function(s)
calcium ion binding protein binding myosin heavy chain binding metal ion binding
Process(es)
muscle contraction regulation of cell shape platelet aggregation
It has to be done as per old AB suggested Products section.
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