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  • CCL20 Antibodies

Invitrogen

MIP-3 alpha Monoclonal Antibody (114908)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite MIP-3 alpha Monoclonal Antibody (114908)

  • Antibody Testing Data (1)
MIP-3 alpha Antibody in Neutralization (Neu)
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MIP-3 alpha Antibody in Neutralization (Neu)
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MIP-3 alpha Antibody (MA5-23857) in Neu

Neutralization of MIP-3 alpha in human CCR6. Samples were incubated in MIP-3 alpha monoclonal antibody (Product # MA5-23857). Recombinant Mouse CCL20/MIP‚3α chemoattracts the BaF3 mouse pro‚B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse CCL20/MIP‚3α (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL20/MIP‚3α Monoclonal Antibody... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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MIP-3 alpha Antibody in Neutralization (Neu)
MIP-3 alpha Monoclonal Antibody (114908)

Product Details

MA5-23857

Applications
Tested Dilution
Publications

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 2-8 µg/mL (Capture)
-

Neutralization (Neu)

3-15 µg/mL
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

114908

Immunogen

E. coli-derived recombinant mouse CCL20/MIP-3 alpha Ala28-Met97
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609978

Product Specific Information

This antibody does not cross-react with recombinant human (rh) CCL19, rhCCL20, rmCCL3, 4, 9, 19, rmCXCL2, or rrCCL20.

Reconstitute at 0.5 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

Macrophage Inflammatory Protein-3 alpha (MIP-3alpha), also called CCL20, is expressed in the liver, lungs, lymph nodes and peripheral blood lymphocytes. MIP-3alpha is strongly up regulated by inflammatory signals, and down regulated by the anti-inflammatory cytokine IL-10. MIP3alpha signals through the G protein-coupled receptor, CCR6, and acts as a chemoattractant to lymphocytes and dendritic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: beta chemokine exodus-1; Beta-chemokine exodus-1; C-C motif chemokine 20; CC chemokine LARC; CC chemokine ST38; Liver and activation-regulated chemokine; M-MIP-3-alpha; Macrophage inflammatory protein 3 alpha; MIP 3 a; MIP 3 α; MIP 3alpha; MIP 3α; MIP-3-alpha; MIP3 a; MIP3 alpha; MIP3 α; small inducible cytokine subfamily A20; Small-inducible cytokine A20

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Gene Aliases: Ccl20; CKb4; exodus-1; LARC; MIP-3[a]; MIP-3A; MIP3A; Scya20; ST38

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UniProt ID: (Mouse) O89093

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Entrez Gene ID: (Mouse) 20297

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Function(s)
cytokine activity protein binding chemokine activity
Process(es)
chemotaxis inflammatory response immune response positive regulation of interleukin-1 alpha biosynthetic process positive regulation of nitric-oxide synthase biosynthetic process cell chemotaxis positive regulation of T cell migration
It has to be done as per old AB suggested Products section.
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