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Invitrogen

METAP2 Recombinant Rabbit Monoclonal Antibody (5U2F4)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite METAP2 Recombinant Rabbit Monoclonal Antibody (5U2F4)

  • Antibody Testing Data (2)
METAP2 Antibody in Western Blot (WB)
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METAP2 Antibody in Western Blot (WB)
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METAP2 Antibody (MA5-38071) in WB

Western blot analysis of METAP2 in extracts of various cell lines. Samples were incubated with METAP2 Monoclonal antibody (Product # MA5-38071) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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METAP2 Antibody in Western Blot (WB)
METAP2 Antibody in Western Blot (WB)
METAP2 Recombinant Rabbit Monoclonal Antibody (5U2F4)

Product Details

MA5-38071

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:5,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

5U2F4

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 380-478 of human METAP2 (NP_0068291)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.62 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2897989

Product Specific Information

Positive Samples: Rat testis, DU145, HT-1080, NIH/3T3, C6, Mouse testis

Immunogen sequence: CSHYMKNFDV GHVPIRLPRT KHLLNVINEN FGTLAFCRRW LDRLGESKYL MALKNLCDLG IVDPYPPLCD IKGSYTAQFE HTILLRPTCK EVVSRGDDY

Target Information

The ADP-ribosylation factor (Arf) family are highly conserved members of the Ras superfamily of regulatory GTP-binding proteins. Arf proteins participate in routing of intracellular proteins to and within the Golgi complex. Cellular functions include maintenance of organelle integrity, coat protein assembly, as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: eIF-2-associated p67; eIF-2-associated p67 homolog; initiation factor 2 associated 67 kDa protein; Initiation factor 2-associated 67 kDa glycoprotein; initiation factor 2-associated protein (p67); MAP 2; metAP 2; Methionine aminopeptidase 2; p67; p67eIF2; Peptidase M; peptidase M 2; testicular tissue protein Li 17

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Gene Aliases: 4930584B20Rik; A930035J23Rik; AI047573; AL024412; Amp2; AU014659; MAP2; METAP2; MNPEP; p67; P67EIF2

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UniProt ID: (Human) P50579, (Mouse) O08663, (Rat) P38062

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Entrez Gene ID: (Human) 10988, (Mouse) 56307, (Rat) 64370

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Function(s)
aminopeptidase activity metalloexopeptidase activity poly(A) RNA binding metal ion binding metalloaminopeptidase activity protein binding peptidase activity hydrolase activity
Process(es)
protein processing peptidyl-methionine modification regulation of rhodopsin mediated signaling pathway N-terminal protein amino acid modification protein initiator methionine removal proteolysis
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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