Invitrogen

MEKK2 Recombinant Rabbit Monoclonal Antibody (SC68-02)

Name: MEKK2 Recombinant Rabbit Monoclonal Antibody (SC68-02)
Brand: Invitrogen
SKU: MA5-32237

Advanced Verification

View all (14) MEKK2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (10)
Advanced Verification (1)

MEKK2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

MEKK2 Antibody (MA5-32237) in ICC/IF

Immunofluorescence analysis of Map3k2 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MEKK2 Recombinant Rabbit Monoclonal Antibody (SC68-02) (Product # MA5-32237) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Pl... View More

MEKK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

MEKK2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A549 cells were transfected with Map3k2 siRNA and decrease in signal intensity was observed in Western Blot application using MEKK2 Recombinant Rabbit Monoclonal Antibody (SC68-02) (Product # MA5-32237). {KD}

Immunogen image

Immunogen Image MEKK2 Recombinant Rabbit Monoclonal Antibody (SC68-02)

Product Details

MA5-32237

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:50-1:200

Flow Cytometry (Flow)

1:50-1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SC68-02

Immunogen

Synthetic peptide within Human MEKK2 aa 1-47

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2809523

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

MAP3K2 (MEKK2) is a serine/threonine kinase that activates kinases involved in the MAP kinase signaling pathway. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: 2.7.11.25; MAP/ERK kinase kinase 2; MAPK/ERK kinase kinase 2; MEK kinase 2; MEKK 2; mitogen activated protein kinase kinase kinase 2; Mitogen-activated protein kinase kinase kinase 2; protein kinase MEKK2b

View more View less

Gene Aliases: 9630061B06Rik; AI585793; MAP3K2; MAPKKK2; MEKK2; MEKK2B

View more View less

UniProt ID: (Human) Q9Y2U5

View more View less

Entrez Gene ID: (Human) 10746, (Rat) 171492, (Mouse) 26405

View more View less

Function(s)

protein kinase activity protein serine/threonine kinase activity receptor signaling protein serine/threonine kinase activity MAP kinase kinase kinase activity protein binding ATP binding protein kinase binding metal ion binding MAP kinase kinase activity nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

MAPK cascade activation of MAPKK activity activation of MAPK activity protein phosphorylation cell proliferation positive regulation of transcription, DNA-templated cellular response to mechanical stimulus phosphorylation activation of JUN kinase activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

MEKK2 Recombinant Rabbit Monoclonal Antibody (SC68-02)

MEKK2 Antibody (MA5-32237) in ICC/IF

Product Details

MA5-32237

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!