Bethyl Laboratories

MED12 Polyclonal Antibody

View all (15) MED12 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers
MED12 Polyclonal Antibody

Product Details

A300-774A

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000-1:10,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000 to 1:5,000
-

Immunoprecipitation (IP)

2-10 µg/mg lysate
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 2127 and the C-terminus (residue 2177) of human Mediator of RNA Polymerase II Transcription, subunit 12.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

tris citrate/phosphate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Based on 100% sequence identity, this antibody is predicted to react with Chimpanzee

Target Information

The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Activator-recruited cofactor 240 kDa component; ARC240; CAG repeat protein 45; human opposite paired; Mediator complex subunit 12; Mediator of RNA polymerase II transcription subunit 12; mediator of RNA polymerase II transcription, subunit 12 homolog; OPA-containing protein; OPA-containing protein 1; putative mediator subunit 12; Thyroid hormone receptor-associated protein complex 230 kDa component; thyroid hormone receptor-associated protein, 230 kDa subunit; Trap230; trinucleotide repeat containing 11 (THR-associated protein); trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit); Trinucleotide repeat-containing gene 11 protein

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Gene Aliases: 230kDa; ARC240; CAGH45; FGS1; HOPA; KIAA0192; MED12; MED12S; Mopa; OHDOX; OKS; OPA-1; OPA1; TNRC11; TRAP230

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UniProt ID: (Human) Q93074, (Mouse) A2AGH6

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Entrez Gene ID: (Human) 9968, (Mouse) 59024

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Function(s)
RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription cofactor activity RNA polymerase II transcription coactivator activity chromatin binding transcription cofactor activity transcription coactivator activity receptor activity protein binding beta-catenin binding protein C-terminus binding protein domain specific binding ligand-dependent nuclear receptor transcription coactivator activity vitamin D receptor binding thyroid hormone receptor binding ubiquitin protein ligase activity transcriptional activator activity, RNA polymerase II transcription factor binding transcription factor binding
Process(es)
neural tube closure transcription initiation from RNA polymerase II promoter endoderm development heart development oligodendrocyte development Schwann cell development protein ubiquitination stem cell population maintenance spinal cord development negative regulation of Wnt signaling pathway intracellular steroid hormone receptor signaling pathway androgen receptor signaling pathway post-anal tail morphogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic neurocranium morphogenesis canonical Wnt signaling pathway Wnt signaling pathway, planar cell polarity pathway axis elongation involved in somitogenesis embryonic brain development transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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