Invitrogen

Leptin Polyclonal Antibody, Biotin, PeproTech®

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Datasheet
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Questions & Answers
Leptin Polyclonal Antibody, Biotin, PeproTech®

Product Details

500-P185GBT-25UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.25-1.0 µg/mL
-
Product Specifications

Species Reactivity

Rat

Host/Isotype

Goat

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Rat Leptin
View immunogen

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929712

Product Specific Information

AA Sequence of recombinant protein: MVPIHKVQDD TKTLIKTIVT RINDISHTQS VSARQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQILTSLP SQNVLQIAHD LENLRDLLHL LAFSKSCSLP QTRGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDLSPEC.

Preparation: Produced from sera of goats immunized with highly pure Recombinant Rat Leptin. Anti-Rat Leptin-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Rat Leptin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat Leptin (500-P185G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat Leptin.

Western Blot: To detect Rat Leptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat Leptin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P185GBT-1MG will be provided as 2 x 500 µg

Target Information

Leptin is a 167 amino acid long protein hormone with important effects in regulating body weight, metabolism and reproductive function. Leptin is approximately 16 kDa in mass and encoded by the obese (ob) gene. Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells. Leptin's effects on body weight are mediated through effects on hypothalamic centers that control feeding behavior and hunger, body temperature and energy expenditure. Leptin is involved in regulating food intake, energy expenditure, and adiposity through hypothalamic leptin receptors. Leptin promotes hematopoiesis, angiogenesis, wound healing, inflammation, immune responses, influences pubertal development and fetal growth. Studies have investigated the role of leptin in obesity, anorexia nervosa, insulin resistance, and hypertension. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans can be caused by defects in leptin production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD295 antigen; H-Leptin; HuB219; LEP-R; Leptin; OB-R; obesity; Obesity factor; obesity protein

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Gene Aliases: Lep; OB; obese

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UniProt ID: (Rat) P50596

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Entrez Gene ID: (Rat) 25608

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Function(s)
cytokine activity hormone activity growth factor activity peptide hormone receptor binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter ovulation from ovarian follicle response to hypoxia positive regulation of cytokine production placenta development regulation of protein phosphorylation response to dietary excess cardiac muscle hypertrophy glucose metabolic process regulation of gluconeogenesis energy reserve metabolic process glycerol biosynthetic process lipid metabolic process fatty acid beta-oxidation JAK-STAT cascade tyrosine phosphorylation of STAT protein female pregnancy response to nutrient circadian rhythm cholesterol metabolic process bile acid metabolic process regulation of blood pressure positive regulation of cell proliferation adult feeding behavior fatty acid catabolic process negative regulation of metabolic process regulation of metabolic process cAMP-mediated signaling central nervous system neuron development insulin secretion regulation of intestinal cholesterol absorption negative regulation of appetite response to insulin response to vitamin E leptin-mediated signaling pathway positive regulation of luteinizing hormone secretion positive regulation of peroxisome proliferator activated receptor signaling pathway intracellular signal transduction bone mineralization involved in bone maturation positive regulation of protein import into nucleus hormone metabolic process positive regulation of tyrosine phosphorylation of Stat3 protein glucose homeostasis eating behavior negative regulation of apoptotic process positive regulation of ion transport positive regulation of MAPK cascade regulation of fat cell differentiation positive regulation of myeloid cell differentiation negative regulation of vasoconstriction positive regulation of JAK-STAT cascade positive regulation of insulin receptor signaling pathway positive regulation of follicle-stimulating hormone secretion positive regulation of developmental growth regulation of insulin secretion regulation of steroid biosynthetic process leukocyte tethering or rolling regulation of lipoprotein lipid oxidation adipose tissue development negative regulation of cartilage development negative regulation of glucagon secretion cellular response to L-ascorbic acid cellular response to retinoic acid regulation of protein localization to nucleus positive regulation of STAT protein import into nucleus positive regulation of reactive oxygen species metabolic process negative regulation of glutamine transport positive regulation of hepatic stellate cell activation
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