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  • Primary Antibodies ›
  • LOXL2 Antibodies

Invitrogen

LOXL2 Recombinant Rabbit Monoclonal Antibody (2E5)

View all (29) LOXL2 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite LOXL2 Recombinant Rabbit Monoclonal Antibody (2E5)

  • Antibody Testing Data (5)
LOXL2 Antibody in Immunocytochemistry (ICC/IF)
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LOXL2 Antibody in Immunocytochemistry (ICC/IF)
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LOXL2 Antibody (MA5-49867) in ICC/IF

Immunocytochemistry analysis of HepG2 cells with LOXL2 monoclonal antibody (Product # MA5-49867) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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LOXL2 Antibody in Immunocytochemistry (ICC/IF)
LOXL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
LOXL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
LOXL2 Antibody in Western Blot (WB)
LOXL2 Antibody in Flow Cytometry (Flow)

Product Details

MA5-49867

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:5,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:20-1:200
-

Flow Cytometry (Flow)

1:20-1:200
-

ELISA (ELISA)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

2E5

Immunogen

A synthesized peptide derived from human LOXL2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3094115

Target Information

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Lysyl oxidase homolog 2; lysyl oxidase related 2; lysyl oxidase-like 2 delta e13; lysyl oxidase-like 2 protein; Lysyl oxidase-like protein 2; Lysyl oxidase-related protein 2; Lysyl oxidase-related protein WS9-14

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Gene Aliases: 1110004B06Rik; 4930526G11Rik; 9430067E15Rik; LOR2; LOXL2; WS9-14

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UniProt ID: (Human) Q9Y4K0, (Mouse) P58022, (Rat) B5DF27

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Entrez Gene ID: (Human) 4017, (Mouse) 94352, (Rat) 290350

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Function(s)
chromatin binding transcription corepressor activity protein-lysine 6-oxidase activity scavenger receptor activity copper ion binding protein binding electron carrier activity methylated histone binding oligosaccharide binding oxidoreductase activity oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor metal ion binding
Process(es)
response to hypoxia epithelial to mesenchymal transition endothelial cell proliferation sprouting angiogenesis transcription, DNA-templated regulation of transcription, DNA-templated cellular protein modification process chromatin modification histone modification protein deamination collagen fibril organization positive regulation of chondrocyte differentiation endothelial cell migration negative regulation of transcription, DNA-templated response to copper ion oxidation-reduction process receptor-mediated endocytosis cell adhesion aging
It has to be done as per old AB suggested Products section.
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