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LILRB1 Monoclonal Antibody (3D3-1D12)

View all (43) CD85j (ILT2) antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

H00010859-M01

Applications
Tested Dilution
Publications

Western Blot (WB)

1-5 µg/mL
-

ELISA (ELISA)

10 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

3D3-1D12

Immunogen

LILRB1 (AAH15731, 18 a.a. approximately 650 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

Product may be used with Western Blot (Cell lysate).

Immunogen sequence: RTHVQAGHLP KPTLWAEPGS VITQGSPVTL RCQGGQETQE YRLYREKKTA PWITRIPQEL VKKGQFPIPS ITWEHAGRYR CYYGSDTAGR SESSDPLELV VTGAYIKPTL SAQPSPVVNS GGNVTLQCDS QVAFDGFILC KEGEDEHPQC LNSQPHARGS SRAIFSVGPV SPSRRWWYRC YAYDSNSPYE WSLPSDLLEL LVLGVSKKPS LSVQPGPIVA PEETLTLQCG SDAGYNRFVL YKDGERDFLQ LAGAQPQAGL SQANFTLGPV SRSYGGQYRC YGAHNLSSEW SAPSDPLDIL IAGQFYDRVS LSVQPGPTVA SGENVTLLCQ SQGWMQTFLL TKEGAADDPW RLRSTYQSQK YQAEFPMGPV TSAHAGTYRC YGSQSSKPYL LTHPSDPLEL VVSGPSGGPS SPTTGPTSTS GPEDQPLTPT GSDPQSGLGR HLGVVIGILV AVILLLLLLL LLFLILRHRR QGKHWTSTQR KADFQHPAGA VGPEPTDRGL QWRSSPAADA QEENLYAAVK HTQPEDGVEM DTRSPHDEDP QAVTYAEVKH SRPRREMASP PSPLSGEFLD TKDRQAEEDR QMDTEAAASE APQDVTYAQL HSLTLRRKAT EPPPSQEGPS PAVPSIYATL AIH

Target Information

CD85j, also known as a member of the leukocyte immunoglobulin-like receptor (LIR) family, is a single transmembrane glycoprotein with a long cytoplasmic domain containing four immunoreceptor tyrosine-based inhibitory motifs (ITIMs). These motifs signal through interactions with SHP-1, transducing negative signals that inhibit immune response stimulation. CD85j is expressed on myeloid cells such as monocytes and dendritic cells, as well as on subsets of lymphoid cells including NK, T, and B cells. The expression of CD85j is correlated with leukemias such as acute lymphoblastic leukemia (ALL) and chronic lymphocytic leukemia (CLL). On CD8+ cells, CD85j expression is associated with effector cell function and plays a significant role in viral infections, including HIV, Epstein-Barr virus, and cytomegalovirus (CMV). CD85j binds to MHC class I molecules, including HLA-G, A, F, B27, E, and F, on antigen-presenting cells. The receptor helps control inflammatory responses and cytotoxicity, focusing the immune response and limiting autoreactivity. CD85j belongs to the subfamily B class of LIR receptors, which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and multiple transcript variants encoding different isoforms have been identified for this gene. Its gene is located in a cluster at chromosomal region 19q13.4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD85 antigen-like family member J; CD85j; Ig-like transcript 2; ILT-2; Immunoglobulin-like transcript 2; leucocyte Ig-like receptor B1; Leukocyte immunoglobulin-like receptor subfamily B member 1; leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1; LIR-1; MIR-7; Monocyte/macrophage immunoglobulin-like receptor 7; myeloid inhibitory receptor 7

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Gene Aliases: CD85J; ILT-2; ILT2; LILRB1; LIR-1; LIR1; MIR-7; MIR7; PIR-B; PIRB

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UniProt ID: (Human) Q8NHL6

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Entrez Gene ID: (Human) 10859

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Function(s)
protein phosphatase 1 binding HLA-A specific inhibitory MHC class I receptor activity HLA-B specific inhibitory MHC class I receptor activity MHC class I receptor activity SH2 domain binding MHC class I protein binding protein homodimerization activity
Process(es)
negative regulation of T cell mediated cytotoxicity positive regulation of defense response to virus by host adaptive immune response T cell proliferation involved in immune response negative regulation of cytokine secretion involved in immune response immune response-inhibiting cell surface receptor signaling pathway Fc receptor mediated inhibitory signaling pathway signal transduction response to virus positive regulation of gene expression negative regulation of serotonin secretion receptor internalization interferon-gamma production negative regulation of interferon-gamma production negative regulation of mononuclear cell proliferation negative regulation of interferon-beta secretion negative regulation of T cell proliferation negative regulation of tumor necrosis factor biosynthetic process positive regulation of apoptotic process negative regulation of interferon-gamma biosynthetic process negative regulation of cell cycle negative regulation of endocytosis positive regulation of cytolysis positive regulation of transcription from RNA polymerase II promoter negative regulation of natural killer cell mediated cytotoxicity negative regulation of alpha-beta T cell activation regulation of immune response defense response to virus negative regulation of calcium ion transport cellular response to lipopolysaccharide interferon-gamma secretion dendritic cell differentiation negative regulation of dendritic cell apoptotic process negative regulation of interleukin-10 secretion negative regulation of interleukin-12 secretion negative regulation of CD8-positive, alpha-beta T cell activation negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell positive regulation of gamma-delta T cell activation involved in immune response negative regulation of dendritic cell differentiation negative regulation of transforming growth factor-beta secretion negative regulation of osteoclast development
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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