Invitrogen

Ku70 Polyclonal Antibody

Name: Ku70 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-83871

Advanced Verification

View all (34) Ku70 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Advanced Verification (2)

Ku70 Antibody in Immunocytochemistry (ICC/IF)

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Ku70 Antibody (PA5-83871) in ICC/IF

Immunofluorecent analysis of Ku70 in human cell line HEK 293 using Ku70 Polyclonal Antibody (Product # PA5-83871). Staining shows localization to nucleoplasm.

Ku70 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Ku70 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A-431 cells were transfected with, control siRNA, Ku70 siRNA probe #1, and Ku70 siRNA probe #2 and a decrease in signal intensity was observed in Western Blot application using anti-Ku70 Polyclonal Antibody (Product # PA5-83871) used at a dilution of 0.4 µg/mL. Anti-GAPDH was used as a loading control. {KD}

Immunogen image

Immunogen Image Ku70 Polyclonal Antibody

Product Details

PA5-83871

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000-1:2,500

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human XRCC6. Recombinant protein control fragment (Product #RP-103007).

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2791023

Product Specific Information

Immunogen sequence: PPYFVALVPQ EEELDDQKIQ VTPPGFQLVF LPFADDKRKM PFTEKIMATP EQVGKMKAIV EKLRFTYRSD SFENPVLQQH FRNLEALALD LMEPEQAVDL

Target Information

XRCC6 is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; ATP-dependent DNA helicase II, 70 kDa subunit; CT; CTA-216E10.7; CTC box binding factor 75 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; DNA repair protein XRCC6; Ku autoantigen p70 subunit; Ku autoantigen, 70kDa; Ku70; Lupus Ku autoantigen protein p70; OTTHUMP00000028581; thyroid autoantigen 70kD (Ku antigen); thyroid autoantigen 70kDa (Ku antigen); Thyroid-lupus autoantigen; thyroid-lupus autoantigen p70; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 6; X-ray repair cross-complementing protein 6

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Gene Aliases: CTC75; CTCBF; G22P1; KU70; ML8; TLAA; XRCC6

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UniProt ID: (Human) P12956

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Entrez Gene ID: (Human) 2547

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Function(s)

DNA binding damaged DNA binding double-stranded DNA binding double-stranded telomeric DNA binding ATP-dependent DNA helicase activity protein binding ATP binding protein C-terminus binding transcription regulatory region DNA binding poly(A) RNA binding macromolecular complex binding 5'-deoxyribose-5-phosphate lyase activity

Process(es)

telomere maintenance DNA ligation double-strand break repair via nonhomologous end joining DNA recombination transcription, DNA-templated positive regulation of type I interferon production DNA duplex unwinding negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of smooth muscle cell proliferation protein heterotetramerization cellular response to gamma radiation cellular response to X-ray establishment of integrated proviral latency double-strand break repair via classical nonhomologous end joining

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Ku70 Polyclonal Antibody

Ku70 Antibody (PA5-83871) in ICC/IF

Product Details

PA5-83871

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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