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  • Ku70 Antibodies

Bethyl Laboratories

Ku70 Polyclonal Antibody

View all (34) Ku70 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite Ku70 Polyclonal Antibody

  • Antibody Testing Data (2)
Ku70 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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Ku70 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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Ku70 Antibody (A302-625A) in IHC (P)

Detection of human Ku70 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Ku70 (Product # A302-625A) (Lot 1) used at a dilution of 1:200 (1 µg/mL). Detection: DAB. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Ku70 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Ku70 Antibody in Western Blot (WB)
Ku70 Polyclonal Antibody

Product Details

A302-625A

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000-1:10,000
-

Immunohistochemistry (IHC)

1:200-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000
-

Immunoprecipitation (IP)

2-5 µg/mg lysate
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 559 and 609 of human Lupus Ku autoantigen protein p70.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.20 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.0 to 8.0, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

XRCC6 is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; ATP-dependent DNA helicase II, 70 kDa subunit; CT; CTA-216E10.7; CTC box binding factor 75 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; DNA repair protein XRCC6; Ku autoantigen p70 subunit; Ku autoantigen, 70kDa; Ku70; Lupus Ku autoantigen protein p70; OTTHUMP00000028581; thyroid autoantigen 70kD (Ku antigen); thyroid autoantigen 70kDa (Ku antigen); Thyroid-lupus autoantigen; thyroid-lupus autoantigen p70; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 6; X-ray repair cross-complementing protein 6

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Gene Aliases: CTC75; CTCBF; G22P1; KU70; ML8; TLAA; XRCC6

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UniProt ID: (Human) P12956

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Entrez Gene ID: (Human) 2547

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Function(s)
DNA binding damaged DNA binding double-stranded DNA binding double-stranded telomeric DNA binding ATP-dependent DNA helicase activity protein binding ATP binding protein C-terminus binding transcription regulatory region DNA binding poly(A) RNA binding macromolecular complex binding 5'-deoxyribose-5-phosphate lyase activity
Process(es)
telomere maintenance DNA ligation double-strand break repair via nonhomologous end joining DNA recombination transcription, DNA-templated positive regulation of type I interferon production DNA duplex unwinding negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of smooth muscle cell proliferation protein heterotetramerization cellular response to gamma radiation cellular response to X-ray establishment of integrated proviral latency double-strand break repair via classical nonhomologous end joining
It has to be done as per old AB suggested Products section.
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