Bioss

Klotho Polyclonal Antibody

Name: Klotho Polyclonal Antibody
Brand: Bioss
SKU: BS-2925R

View all (16) Klotho antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)

Klotho Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 5

Klotho Antibody (BS-2925R) in IHC (P)

Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:400 overnight at 4°C, followed by a conjugated secondary and D... View More

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Immunogen image

Immunogen Image Klotho Polyclonal Antibody

Product Details

BS-2925R

Applications

Tested Dilution

Publications

Western Blot (WB)

1:300

Immunohistochemistry (Paraffin) (IHC (P))

1:400

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500

Immunocytochemistry (ICC/IF)

1:50-1:200

ELISA (ELISA)

1:500-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide derived from human Klotho, amino acids 401-500.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

KLOTHO is the systemic anti-aging hormone within the glycosidase1 superfamily. It encodes a type I membrane protein that is abundant in the kidney and brain. In mice, a deficiency in KLOTHO expression leads to various systemic phenotypes resembling human aging such as arteriosclerosis, osteoporosis, and skin atrophy together with growth retardation, short life-span and infertility. Transgenic mice overexpressing KLOTHO have an extended life span by inhibiting insulin/IGF1 signaling. KLOTHO is involved in the regulation of calcium/phosphorus homeostasis by inhibiting the synthesis of active vitamin D and identified as a potential tumor suppressor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: HFTC3; Klotho; secreted form of Klotho protein

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Gene Aliases: alpha-kl; KL

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UniProt ID: (Human) Q9UEF7, (Rat) Q9Z2Y9, (Mouse) O35082

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Entrez Gene ID: (Human) 9365, (Rat) 83504, (Mouse) 16591

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Function(s)

beta-glucuronidase activity signal transducer activity Ras guanyl-nucleotide exchange factor activity fibroblast growth factor receptor binding hormone activity vitamin D binding beta-glucosidase activity 1-phosphatidylinositol-3-kinase activity fibroblast growth factor binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity hydrolase activity, hydrolyzing O-glycosyl compounds protein binding hydrolase activity hydrolase activity, acting on glycosyl bonds

Process(es)

acute inflammatory response carbohydrate metabolic process energy reserve metabolic process aging insulin receptor signaling pathway positive regulation of bone mineralization calcium ion homeostasis positive regulation of MAPKKK cascade by fibroblast growth factor receptor signaling pathway metabolic process fibroblast growth factor receptor signaling pathway MAPK cascade regulation of phosphatidylinositol 3-kinase signaling phosphatidylinositol-3-phosphate biosynthetic process positive regulation of GTPase activity phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling glycosyl compound metabolic process

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Klotho Polyclonal Antibody

Klotho Antibody (BS-2925R) in IHC (P)

Product Details

BS-2925R

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Target Information

References (0)

Bioinformatics

Performance Guarantee

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