Invitrogen

KLF2 Monoclonal Antibody (OTI6A3)

Name: KLF2 Monoclonal Antibody (OTI6A3)
Brand: Invitrogen
SKU: MA5-26825

Advanced Verification

2 Published Figures

1 Reference

View all (44) KLF2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

KLF2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

KLF2 Antibody (MA5-26825) in ICC/IF

Immunofluorescence analysis of Krueppel-like factor 2 was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with KLF2 Monoclonal Antibody (OTI6A3) (Product # MA5-26825) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor... View More

KLF2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

KLF2 Antibody — Detection of altered subcellular localization of the target protein by cell treatment demonstrates antibody specificity. Immunofluorescence analysis using KLF2 Monoclonal Antibody (OTI6A3) (Product # MA5-26825), shows Shows increased expression upon Rapamycin treatment and decreased expression upon TNF Alpha treatment in HUVEC cells.(Ref :https://doi.org/10.1161/01.RES.0000159707.05637.a1, https://doi.org/10.1371/journal.pone.0043315). {TM}

Immunogen image

Immunogen Image KLF2 Monoclonal Antibody (OTI6A3)

Product Details

MA5-26825

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI6A3

Immunogen

Full length human recombinant protein of KLF2 produced in E.coli

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2723312

Target Information

KLF2 belongs to the krueppel C2H2-type zinc-finger protein family. It contains 3 C2H2-type zinc fingers. KLF2 binds to the CACCC box in the beta-globin gene promoter and activates transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Krueppel-like factor 2; Kruppel-like factor 2 (lung); Kruppel-like factor LKLF; Lung krueppel-like factor; lung Kruppel-like factor; lung Kruppel-like zinc finger transcription factor

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Gene Aliases: KLF2; LKLF

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UniProt ID: (Human) Q9Y5W3

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Entrez Gene ID: (Human) 10365

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Function(s)

transcription factor activity, sequence-specific DNA binding protein binding sequence-specific DNA binding metal ion binding

Process(es)

cell morphogenesis in utero embryonic development transcription, DNA-templated negative regulation of interleukin-6 production multicellular organism growth positive regulation of transcription from RNA polymerase II promoter in response to stress regulation of gene expression, epigenetic erythrocyte maturation positive regulation of nitric oxide biosynthetic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of protein metabolic process Type I pneumocyte differentiation cellular response to hydrogen peroxide cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to cycloheximide cellular response to laminar fluid shear stress cellular response to peptide

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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KLF2 Monoclonal Antibody (OTI6A3)

KLF2 Antibody (MA5-26825) in ICC/IF

Product Details

MA5-26825

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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