Invitrogen

KHK Monoclonal Antibody (OTI1D8)

Name: KHK Monoclonal Antibody (OTI1D8)
Brand: Invitrogen
SKU: MA5-25136

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View all (34) KHK antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (11)
Advanced Verification (1)

KHK Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

KHK Antibody (MA5-25136) in ICC/IF

Immunofluorescence analysis of KHK was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with KHK Monoclonal Antibody (OTI1D8) (Product # MA5-25136) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), ... View More

KHK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

KHK Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with Ketohexokinase siRNA and decrease in signal intensity was observed in Western Blot application using Anti-KHK Monoclonal Antibody (OTI1D8) (Product # MA5-25136). {KD}

Immunogen image

Immunogen Image KHK Monoclonal Antibody (OTI1D8)

Product Details

MA5-25136

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50

Immunocytochemistry (ICC/IF)

1:100

Flow Cytometry (Flow)

1:100

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI1D8

Immunogen

Full length human recombinant protein of KHK produced in HEK293T cell

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2724561

Target Information

KHK encodes the gene ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The splice variant presented encodes the highly active form found in liver. renal cortex. and small intestine. while the alternate variant encodes the lowe

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: fructokinase; Hepatic fructokinase; Ketohexokinase; ketohexokinase (fructokinase); testicular tissue protein Li 102

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Gene Aliases: KHK

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UniProt ID: (Human) P50053

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Entrez Gene ID: (Human) 3795

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Function(s)

ketohexokinase activity protein binding ATP binding

Process(es)

response to sucrose response to glucose response to fructose response to zinc ion response to insulin carbohydrate phosphorylation fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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KHK Monoclonal Antibody (OTI1D8)

KHK Antibody (MA5-25136) in ICC/IF

Product Details

MA5-25136

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

References (0)

Bioinformatics

Performance Guarantee

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