Invitrogen

KHK Polyclonal Antibody

Name: KHK Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-29004

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View all (34) KHK antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (9)
Advanced Verification (2)

KHK Antibody in Immunocytochemistry (ICC/IF)

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KHK Antibody (PA5-29004) in ICC/IF

Immunofluorescence analysis of KHK was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with KHK Polyclonal Antibody (Product # PA5-29004) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), 1:2000 d... View More

KHK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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KHK Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues tested owing to their inherent genetic constitution. Relative expression of Ketohexokinase was observed to be high in Mouse Liver, Rat Liver, Mouse kidney and Rat Kidney in comparison to low or negative in Mouse Lung, Rat Lung, Mouse Brain, Rat Brain and Mouse Skeletal Muscle using Anti-KHK Polyclonal Antibody (Product # PA5-29004) in Western Blot (Ref :DOI: 10.12688/f1000research.17082.2) {RE}

Immunogen image

Immunogen Image KHK Polyclonal Antibody

Product Details

PA5-29004

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Full length human Ketohexokinase Recombinant protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2546480

Product Specific Information

Recommended positive controls: HeLa, HepG2, mouse Liver.

Predicted reactivity: Mouse (87%), Rat (86%), Dog (85%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

KHK encodes the gene ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The splice variant presented encodes the highly active form found in liver. renal cortex. and small intestine. while the alternate variant encodes the lowe

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: fructokinase; Hepatic fructokinase; Ketohexokinase; ketohexokinase (fructokinase); testicular tissue protein Li 102

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Gene Aliases: KETHPRO; KHK

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UniProt ID: (Human) P50053, (Mouse) P97328, (Rat) Q02974

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Entrez Gene ID: (Human) 3795, (Mouse) 16548, (Rat) 25659

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Function(s)

ketohexokinase activity protein binding ATP binding nucleotide binding fructokinase activity kinase activity transferase activity

Process(es)

carbohydrate metabolic process fructose metabolic process response to sucrose response to glucose response to zinc ion phosphorylation response to insulin carbohydrate phosphorylation regulation of glycogen metabolic process response to fructose fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate carbohydrate catabolic process

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KHK Polyclonal Antibody

KHK Antibody (PA5-29004) in ICC/IF

Product Details

PA5-29004

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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