Invitrogen

KDEL Monoclonal Antibody (2D6), APC

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Datasheet
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Datasheet
Protocols
Questions & Answers
KDEL Monoclonal Antibody (2D6), APC

Product Details

MA5-45873

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2D6

Immunogen

KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)
View immunogen

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2932327

Product Specific Information

A 1:1,000 dilution of MA5-45873 was sufficient for detection of KDEL in 10 µg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects KDEL tagged proteins.

Target Information

The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins, GRP78 and GRP94 respectively, and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 78 kDa glucose-regulated protein; 94 kDa glucose-regulated protein; 94 kDa glucose-regulated protein {ECO:0000250|UniProtKB:P08113}; Binding-immunoglobulin protein; BiP; Endoplasmic reticulum chaperone BiP; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Endoplasmic reticulum resident protein 99; Endoplasmin; endoplasmin {ECO:0000250|UniProtKB:P08113}; endothelial cell (HBMEC) glycoprotein; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; epididymis secretory sperm binding protein Li 89n; ER lumen protein retaining receptor 1; ER lumen protein-retaining receptor 1; ERp99; glucose regulated protein, 78 kDa; glucose-regulated protein, 78kDa; gp96 homolog; GRP 78; GRP-78; GRP-94; heat shock 70 kDa protein 5; heat shock 70kD protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); Heat shock protein 70 family protein 5; Heat shock protein 90 kDa beta member 1; heat shock protein 90 kDa beta member 1 {ECO:0000250|UniProtKB:P08113}; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta family member 1; Heat shock protein family A member 5; Heat shock protein family C member 4; HSP70 family protein 5; hsp90b1 {ECO:0000250|UniProtKB:P08113}; Immunoglobulin heavy chain-binding protein; KDEL; KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1; KDEL endoplasmic reticulum protein retention receptor 1; KDEL receptor 1; Lys-Asp-Glu-Leu; Polymorphic tumor rejection antigen 1; Putative MAPK-activating protein PM23; RP23-446N16.1; Steroidogenesis-activator polypeptide; stress-inducible tumor rejection antigen gp96; transforming growth factor alpha regulated gene 2; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen gp96; XAP-1 antigen

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Gene Aliases: 8030486F04Rik; AL022860; AU019543; AW215843; baffled; BIP; D2Wsu141e; D2Wsu17e; ECGP; endoplasmin; ERD2; ERD2.1; ERp99; GP96; GRP78; GRP94; HDEL; HEL-S-125m; HEL-S-89n; HEL35; Hsce70; HSP90B1; HSPA5; HSPC4; KDELR1; mBiP; MIF2; PM23; SEZ-7; Sez7; TA-3; Targ2; Tra-1; TRA1

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UniProt ID: (Human) P14625, (Human) P11021, (Mouse) P08113, (Rat) Q66HD0, (Rat) P06761, (Mouse) P20029, (Human) P24390, (Mouse) Q99JH8, (Rat) Q569A6

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Entrez Gene ID: (Human) 7184, (Human) 3309, (Mouse) 22027, (Rat) 362862, (Rat) 25617, (Mouse) 14828, (Human) 10945, (Mouse) 68137, (Rat) 361577

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Function(s)
RNA binding calcium ion binding protein binding ATP binding protein phosphatase binding virion binding low-density lipoprotein particle receptor binding unfolded protein binding glycoprotein binding ATPase activity enzyme binding protein domain specific binding ubiquitin protein ligase binding ribosome binding chaperone binding misfolded protein binding cadherin binding involved in cell-cell adhesion nucleotide binding KDEL sequence binding molecular_function ER retention sequence binding
Process(es)
ER overload response activation of signaling protein activity involved in unfolded protein response cerebellum structural organization cerebellar Purkinje cell layer development substantia nigra development positive regulation of cell migration negative regulation of transforming growth factor beta receptor signaling pathway positive regulation of protein ubiquitination response to endoplasmic reticulum stress maintenance of protein localization in endoplasmic reticulum cellular response to glucose starvation negative regulation of apoptotic process proteolysis involved in cellular protein catabolic process cellular response to antibiotic cellular response to interleukin-4 toxin transport response to hypoxia toll-like receptor signaling pathway receptor-mediated endocytosis protein transport ER-associated ubiquitin-dependent protein catabolic process retrograde protein transport, ER to cytosol actin rod assembly protein folding in endoplasmic reticulum ATF6-mediated unfolded protein response regulation of phosphoprotein phosphatase activity sequestering of calcium ion cellular response to ATP positive regulation of embryonic development protein retention in ER lumen intracellular protein transport ER to Golgi vesicle-mediated transport retrograde vesicle-mediated transport, Golgi to ER protein folding response to stress transport biological_process vesicle-mediated transport endoplasmic reticulum unfolded protein response IRE1-mediated unfolded protein response PERK-mediated unfolded protein response regulation of protein folding in endoplasmic reticulum cellular response to manganese ion negative regulation of protein homodimerization activity cell-cell adhesion regulation of ATF6-mediated unfolded protein response regulation of IRE1-mediated unfolded protein response regulation of PERK-mediated unfolded protein response positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress
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