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Insulin-like Growth Factor-1 (IGF-1) Monoclonal Antibody (IGF1, 1020)

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Datasheet
Protocols
Questions & Answers

Cite Insulin-like Growth Factor-1 (IGF-1) Monoclonal Antibody (IGF1, 1020)

  • Antibody Testing Data (1)
Insulin-like Growth Factor-1 (IGF-1) Antibody in Immunocytochemistry (ICC/IF)
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Insulin-like Growth Factor-1 (IGF-1) Antibody in Immunocytochemistry (ICC/IF)
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Insulin-like Growth Factor-1 (IGF-1) Antibody (3479-MSM2-P1ABX) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis of HeLa cells using Insulin-like Growth Factor-1 (IGF-1) Monoclonal Antibody (Product # 3479-MSM2-P1ABX) (Green). Nuclei are counter stained with DAPI (Blue).. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Insulin-like Growth Factor-1 (IGF-1) Antibody in Immunocytochemistry (ICC/IF)
Insulin-like Growth Factor-1 (IGF-1) Monoclonal Antibody (IGF1, 1020)

Product Details

3479-MSM2-P1ABX

Applications
Tested Dilution
Publications

Immunocytochemistry (ICC/IF)

1-4 µg/mL
-

Flow Cytometry (Flow)

1-2 µg/10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Rabbit, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

IGF1, 1020

Immunogen

Recombinant full-length human IGF-1 protein
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

IGF1 (Insulin-like growth factor-1) is structurally and functionally related to insulin but has a much higher growth-promoting activity. A variety of cellular responses are induced by IGF1, including cell proliferation, differentiation, migration, and survival. Further, IGF1 is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types in muscle, bone, and cartilage tissue. IGF1 stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regards to enhancing glucose uptake. In circulation, IGFs are predominantly bound to binding proteins (IGFBPs) which prolong the half-life of the IGFs and play a role in delivering them to target tissues. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Moreover, mature human IGF-I is 100% homologous with bovine and porcine proteins. Low levels of IGF1 have been linked to Alzheimer's disease. IGF1 is processed from a precursor, bound by a specific receptor, and secreted. Defects in the IGF1 gene are a cause of insulin-like growth factor 1 deficiency and several transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: H-IGF-1; IGF; IGF-I; IGF-IA; IGF-IB; IGF1A; IGFIa; Insulin like growth factor; Insulin-like growth factor 1; insulin-like growth factor 1 (somatomedin C); Insulin-like growth factor I; insulin-like growth factor IB; M-IGF-1; Mechano growth factor; MGF; OTTHUMP00000195084; R-IGF-1; Somatomedin; Somatomedin C; Somatomedin-C

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Gene Aliases: C730016P09Rik; IBP1; IGF-1; IGF-I; IGF-IB; IGF1; IGFI; MGF

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UniProt ID: (Human) P05019, (Rabbit) Q95222, (Rat) P08025, (Mouse) P05017

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Entrez Gene ID: (Human) 3479, (Rabbit) 100008668, (Rat) 24482, (Mouse) 16000

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Function(s)
insulin receptor binding insulin-like growth factor receptor binding integrin binding hormone activity steroid binding protein binding growth factor activity protein serine/threonine kinase activator activity
Process(es)
cell activation regulation of protein phosphorylation blood vessel remodeling regulation of translation nervous system development female pregnancy memory cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation response to heat response to mechanical stimulus glial cell differentiation response to organic substance regulation of gene expression positive regulation of cardiac muscle hypertrophy positive regulation of phosphatidylinositol 3-kinase signaling response to organic cyclic compound response to activity skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration muscle hypertrophy myotube cell development positive regulation of smooth muscle cell migration positive regulation of cerebellar granule cell precursor proliferation water homeostasis proteoglycan biosynthetic process positive regulation of cell growth lung development mammary gland development exocrine pancreas development response to nutrient levels response to lipopolysaccharide regulation of establishment or maintenance of cell polarity positive regulation of protein import into nucleus, translocation negative regulation of smooth muscle cell apoptotic process multicellular organism growth bone mineralization involved in bone maturation regulation of multicellular organism growth positive regulation of activated T cell proliferation regulation of cell proliferation positive regulation of tyrosine phosphorylation of Stat5 protein negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of DNA binding positive regulation of MAPK cascade positive regulation of insulin-like growth factor receptor signaling pathway myoblast differentiation positive regulation of fat cell differentiation positive regulation of osteoblast differentiation positive regulation of glycogen biosynthetic process positive regulation of DNA replication positive regulation of glycolytic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import positive regulation of Ras protein signal transduction insulin-like growth factor receptor signaling pathway phosphatidylinositol-mediated signaling positive regulation of fibroblast proliferation lung alveolus development cell development response to steroid hormone positive regulation of smooth muscle cell proliferation branching morphogenesis of an epithelial tube inner ear development chondroitin sulfate proteoglycan biosynthetic process positive regulation of epithelial cell proliferation positive regulation of peptidyl-tyrosine phosphorylation detection of mechanical stimulus involved in sensory perception regulation of protein metabolic process response to glucocorticoid myoblast proliferation positive regulation of protein kinase B signaling regulation of calcium ion transport negative regulation of oocyte development lung vasculature development lung lobe morphogenesis Type I pneumocyte differentiation Type II pneumocyte differentiation prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis prostate gland growth prostate gland epithelium morphogenesis prostate gland stromal morphogenesis negative regulation of androgen receptor signaling pathway negative regulation of ERK1 and ERK2 cascade positive regulation of calcineurin-NFAT signaling cascade cellular response to electrical stimulus positive regulation of protein serine/threonine kinase activity positive regulation of steroid hormone biosynthetic process negative regulation of release of cytochrome c from mitochondria extrinsic apoptotic signaling pathway in absence of ligand negative regulation of neuron death positive regulation of myoblast proliferation negative regulation of extrinsic apoptotic signaling pathway activation of MAPK activity skeletal system development response to hypoxia platelet degranulation DNA replication movement of cell or subcellular component signal transduction Ras protein signal transduction muscle organ development aging response to nutrient glycolate metabolic process positive regulation of glycoprotein biosynthetic process phosphatidylinositol 3-kinase signaling positive regulation of cell migration response to caffeine activation of protein kinase B activity cellular response to insulin stimulus response to nicotine protein kinase B signaling cellular protein metabolic process positive regulation of protein secretion protein stabilization positive regulation of cell growth involved in cardiac muscle cell development ERK1 and ERK2 cascade cellular response to estradiol stimulus positive regulation of trophectodermal cell proliferation negative regulation of cholangiocyte apoptotic process positive regulation of transcription regulatory region DNA binding regulation of intracellular steroid hormone receptor signaling pathway
It has to be done as per old AB suggested Products section.
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