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  • IL-7 Antibodies

Invitrogen

IL-7 Polyclonal Antibody

Advanced Verification
1 Published Figure
1 Reference
View all (42) IL-7 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite IL-7 Polyclonal Antibody

  • Antibody Testing Data (1)
  • Published Figures (1)
  • Advanced Verification (1)
IL-7 Antibody in Neutralization (Neu)
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IL-7 Antibody in Neutralization (Neu)
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IL-7 Antibody (PA5-46944) in Neu

Neutralization of IL-7 in PHA-activated human peripheral blood mononuclear cells (PBMC). Samples were incubated in IL-7 polyclonal antibody (Product # PA5-46944). Recombinant Mouse IL‚7 stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‚7 (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‚7 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.2-0.4 µg/mL. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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IL-7 Antibody in Neutralization (Neu)
IL-7 Antibody in Neutralization (Neu)
IL-7 Antibody
IL-7 Polyclonal Antibody

Product Details

PA5-46944

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1 µg/mL
-

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 0.2-0.8 µg/mL (Capture)
-

Neutralization (Neu)

0.2- 0.4 µg/mL
View 1 publication 1 publication
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived recombinant mouse IL-7 Glu26-Ile154
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609533

Product Specific Information

In sandwich ELISAs, approximately 20% cross-reactivity with recombinant rat IL-7 is observed, and less than 0.1% cross-reactivity with recombinant human IL-7 is observed. This antibody neutralizes the biological activity of recombinant mouse IL-7. This antibody will not neutralize the biological activity of recombinant human IL-7.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

Interleukin 7 (IL-7) is a hematopoietic cytokine that is constitutively produced by stromal cells from the bone marrow and thymus. IL-7 signals through the IL-7R to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD127; H-IL-7; IL-7; IL-7 receptor alpha chain; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; ILN; Interleukin; interleukin 7 receptor alpha chain; Interleukin-7; Interleukin-7 receptor subunit alpha; Interleukin7; M-IL-7

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Gene Aliases: A630026I06Rik; CD127; hlb368; Il-7; IL-7Ralpha; Il7; Il7r

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UniProt ID: (Mouse) P10168, (Mouse) P16872

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Entrez Gene ID: (Mouse) 16196, (Mouse) 16197

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Function(s)
cytokine activity cytokine receptor binding interleukin-7 receptor binding growth factor activity cytokine receptor activity
Process(es)
cell morphogenesis negative regulation of T cell mediated cytotoxicity immunoglobulin production regulation of cell size positive regulation of gene expression cell growth T cell differentiation positive regulation of T cell differentiation in thymus B cell proliferation lymph node development homeostasis of number of cells T cell lineage commitment immune response humoral immune response cell-cell signaling organ morphogenesis regulation of gene expression positive regulation of B cell proliferation negative regulation of catalytic activity bone resorption positive regulation of T cell differentiation positive regulation of organ growth homeostasis of number of cells within a tissue negative regulation of extrinsic apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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