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Invitrogen
IL-7 Polyclonal Antibody, PeproTech®
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FIGURE: 1 / 3
IL-7 Antibody (500-P57-1MG) in ELISA
Product Details
500-P57-1MG
Species Reactivity
Published species
Host/Isotype
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Type
Immunogen
Conjugate
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Product Specific Information
AA Sequence of recombinant protein: MECHIKDKEG KAYESVLMIS IDELDKMTGT DSNCPNNEPN FFRKHVCDDT KEAAFLNRAA RKLKQFLKMN ISEEFNVHLL TVSQGTQTLV NCTSKEEKNV KEQKKNDACF LKRLLREIKT CWNKILKGSI.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-7. Anti-Murine IL-7-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-7 matrix.
Sandwich ELISA: To detect Murine IL-7 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-7 (500-P57Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-7.
Western Blot: To detect Murine IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine IL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Interleukin 7 (IL-7) is a hematopoietic cytokine that is constitutively produced by stromal cells from the bone marrow and thymus. IL-7 signals through the IL-7R to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD127; H-IL-7; IL-7; IL-7 receptor alpha chain; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; ILN; Interleukin; interleukin 7 receptor alpha chain; Interleukin-7; Interleukin-7 receptor subunit alpha; Interleukin7; M-IL-7
Gene Aliases: A630026I06Rik; CD127; hlb368; Il-7; IL-7Ralpha; Il7; Il7r
UniProt ID: (Mouse) P10168, (Mouse) P16872
Entrez Gene ID: (Mouse) 16196, (Mouse) 16197
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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