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  • IL-6 Antibodies

Invitrogen

IL-6 Monoclonal Antibody (MQ2-13A5), eBioscience™

12 Published Figures
22 References
View all (211) IL-6 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite IL-6 Monoclonal Antibody (MQ2-13A5), eBioscience™

  • Published Figures (12)
IL-6 Antibody in ELISA (ELISA)
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IL-6 Antibody in ELISA (ELISA)
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FIGURE: 1 / 12

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IL-6 Antibody (14-7069-81) in ELISA

Scientific reports 2021 - Figure 4 Survival and phagocytosis are reduced in PLCgamma2 Ko iPSC macrophages, while LPS-induced secretion of inflammatory cytokines TNFalpha and IL-6 is unaffected. ( A ) Cell death is slightly increased under normal culture conditions after >= 7 days in the PLCgamma2 Ko line compared to the Parent. n = 3 ( B ) In the absence of M-CSF, PLCgamma2 Ko cells show enhanced sensitivity to cell death, as the pe... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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IL-6 Antibody in Immunocytochemistry (ICC/IF)
IL-6 Antibody in Immunocytochemistry (ICC/IF)
IL-6 Antibody in Flow Cytometry (Flow)
IL-6 Antibody in Flow Cytometry (Flow)
IL-6 Antibody in Flow Cytometry (Flow)
IL-6 Antibody in Flow Cytometry (Flow)
IL-6 Antibody in Flow Cytometry (Flow)
IL-6 Antibody in ELISA (ELISA)
IL-6 Antibody in ELISA (ELISA)
IL-6 Antibody in ELISA (ELISA)
IL-6 Antibody in T-Cell Activation (TCA)
IL-6 Antibody in T-Cell Activation (TCA)

Product Details

14-7069-81

Applications
Tested Dilution
Publications

Immunocytochemistry (ICC/IF)

-
View 1 publication 1 publication

Flow Cytometry (Flow)

-
View 7 publications 7 publications

ELISA (ELISA)

2-8 µg/mL
View 9 publications 9 publications

Neutralization (Neu)

-
View 2 publications 2 publications

Inhibition Assays (IA)

-
View 1 publication 1 publication

T-Cell Activation (TCA)

-
View 1 publication 1 publication

Miscellaneous PubMed (MISC)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

MQ2-13A5

Conjugate

Unconjugated Unconjugated Unconjugated
  • Alexa Fluor 700 
  • APC 
  • Brilliant Violet 650
  • eFluor 450
  • FITC
  • Functional Grade
  • PE 
  • PE-Cyanine7
  • PerCP-eFluor 710
  • Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468426

Product Specific Information

Description: The MQ2-13A5 antibody reacts with human interleukin-6 (IL-6), a 21-28 kDa cytokine secreted by a variety of cell types.

Applications Reported: The MQ2-13A5 antibody has been reported for use in capture of human IL-6 by ELISA, intracellular staining for flow cytometric analysis, and for neutralizing IL-6 bioactivity.

Applications Tested: The MQ2-13A5 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interleukin-6 (IL-6) in combination with the biotin MQ2-39C3 (13-7068) antibody for detection and recombinant human IL-6 (14-8069) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 2-8 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; IFN-beta-2; IL-6; ILN; Interferon beta-2; Interleukin; interleukin BSF-2; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

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Gene Aliases: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6

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UniProt ID: (Human) P05231

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Entrez Gene ID: (Human) 3569

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Function(s)
cytokine activity interleukin-6 receptor binding protein binding growth factor activity
Process(es)
neutrophil apoptotic process response to yeast hepatic immune response neutrophil mediated immunity monocyte chemotaxis positive regulation of acute inflammatory response positive regulation of leukocyte chemotaxis negative regulation of protein kinase activity acute-phase response inflammatory response humoral immune response aging positive regulation of cell proliferation negative regulation of cell proliferation regulation of cell shape response to heat response to cold regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of lipid storage response to auditory stimulus cell growth cytokine-mediated signaling pathway platelet activation response to caffeine endocrine pancreas development neuron projection development response to nutrient levels response to peptidoglycan positive regulation of chemokine production positive regulation of interleukin-6 production response to insulin negative regulation of collagen biosynthetic process positive regulation of peptidyl-serine phosphorylation positive regulation of protein import into nucleus, translocation cellular response to hepatocyte growth factor stimulus positive regulation of T cell proliferation response to drug positive regulation of tyrosine phosphorylation of Stat3 protein glucose homeostasis defense response to protozoan positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process response to amino acid positive regulation of MAPK cascade negative regulation of chemokine biosynthetic process regulation of circadian sleep/wake cycle, non-REM sleep positive regulation of nitric oxide biosynthetic process cell redox homeostasis negative regulation of fat cell differentiation positive regulation of T-helper 2 cell differentiation positive regulation of neuron differentiation positive regulation of osteoblast differentiation negative regulation of gluconeogenesis positive regulation of translation positive regulation of DNA replication regulation of angiogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade response to antibiotic muscle cell cellular homeostasis bone remodeling negative regulation of hormone secretion negative regulation of muscle organ development positive regulation of smooth muscle cell proliferation positive regulation of epithelial cell proliferation negative regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium defense response to Gram-positive bacterium positive regulation of B cell activation positive regulation of immunoglobulin secretion positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity response to glucocorticoid response to calcium ion response to electrical stimulus defense response to virus positive regulation of protein kinase B signaling positive regulation of transmission of nerve impulse branching involved in salivary gland morphogenesis epithelial cell proliferation involved in salivary gland morphogenesis glucagon secretion interleukin-6-mediated signaling pathway cellular response to hydrogen peroxide positive regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to dexamethasone stimulus positive regulation of cell proliferation in bone marrow T-helper 17 cell lineage commitment negative regulation of neuron death positive regulation of STAT protein import into nucleus positive regulation of type B pancreatic cell apoptotic process
It has to be done as per old AB suggested Products section.
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