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  • IL-6 Antibodies

Invitrogen

IL-6 Polyclonal Antibody, PeproTech®

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite IL-6 Polyclonal Antibody, PeproTech®

  • Antibody Testing Data (3)
IL-6 Antibody in ELISA (ELISA)
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IL-6 Antibody in ELISA (ELISA)
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IL-6 Antibody (500-P73G-1MG) in ELISA

Sandwich ELISA: To detect Rat IL-6 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of IL-6 Polyclonal Antibody (Product # 500-P73G-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech IL-6 Polyclonal Antibody, Biotin (Product # 500-P73GBT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-6. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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IL-6 Antibody in Western Blot (WB)
IL-6 Antibody in Western Blot (WB)
IL-6 Antibody in ELISA (ELISA)
IL-6 Polyclonal Antibody, PeproTech®

Product Details

500-P73G-1MG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.5-2.0 µg/mL
-
Product Specifications

Species Reactivity

Rat

Host/Isotype

Goat

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Rat IL-6
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930732

Product Specific Information

AA Sequence of recombinant protein: MFPTSQVRRG DFTEDTTHNR PVYTTSQVGG LITYVLREIL EMRKELCNGN SDCMNSDDAL SENNLKLPEI QRNDGCFQTG YNQEICLLKI CSGLLEFRFY LEFVKNNLQD NKKDKARVIQ SNTETLVHIF KQEIKDSYKI VLPTPTSNAL LMEKLESQKE WLRTKTIQLI LKALEEFLKV TMRSTRQT.

Preparation: Produced from sera of goats immunized with highly pure Recombinant Rat IL-6. Anti-Rat IL-6-specific antibody was purified by affinity chromatography employing an immobilized Rat IL-6 matrix.

Sandwich ELISA: To detect Rat IL-6 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat IL-6 (500-P73GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-6.

Western Blot: To detect Rat IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat IL-6 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: H-IL-6; IL-6; ILN; Interleukin; Interleukin 6 (interferon, beta 2); Interleukin-6; Interleukin6; M-IL-6; R-IL-6

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Gene Aliases: Ifnb2; Il-6; Il6; ILg6

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UniProt ID: (Rat) P20607

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Entrez Gene ID: (Rat) 24498

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Function(s)
receptor binding cytokine activity interleukin-6 receptor binding growth factor activity
Process(es)
neutrophil apoptotic process response to yeast myeloid cell homeostasis hepatic immune response positive regulation of acute inflammatory response negative regulation of protein kinase activity acute-phase response inflammatory response aging positive regulation of cell proliferation negative regulation of cell proliferation regulation of cell shape response to heat response to cold response to wounding response to mechanical stimulus response to bacterium regulation of vascular endothelial growth factor production positive regulation of gene expression response to auditory stimulus response to organic cyclic compound cell growth cytokine-mediated signaling pathway response to caffeine endocrine pancreas development neuron projection development response to nutrient levels response to peptidoglycan response to lipopolysaccharide positive regulation of chemokine production positive regulation of interleukin-6 production response to insulin negative regulation of collagen biosynthetic process positive regulation of peptidyl-serine phosphorylation positive regulation of protein import into nucleus, translocation response to cytokine wound healing positive regulation of T cell proliferation T cell activation regulation of cell proliferation response to drug positive regulation of tyrosine phosphorylation of Stat3 protein glucose homeostasis defense response to protozoan regulation of apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process response to amino acid positive regulation of MAPK cascade negative regulation of chemokine biosynthetic process regulation of circadian sleep/wake cycle, non-REM sleep positive regulation of nitric oxide biosynthetic process cell redox homeostasis positive regulation of T-helper 2 cell differentiation positive regulation of neuron differentiation negative regulation of gluconeogenesis positive regulation of translation positive regulation of DNA replication positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade response to antibiotic muscle cell cellular homeostasis bone remodeling negative regulation of hormone secretion negative regulation of muscle organ development positive regulation of smooth muscle cell proliferation positive regulation of epithelial cell proliferation negative regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium defense response to Gram-positive bacterium positive regulation of B cell activation positive regulation of immunoglobulin secretion positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity response to glucocorticoid response to calcium ion response to electrical stimulus defense response to virus positive regulation of protein kinase B signaling positive regulation of transmission of nerve impulse branching involved in salivary gland morphogenesis epithelial cell proliferation involved in salivary gland morphogenesis glucagon secretion interleukin-6-mediated signaling pathway cellular response to hydrogen peroxide positive regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to dexamethasone stimulus T-helper 17 cell lineage commitment negative regulation of neuron death
It has to be done as per old AB suggested Products section.
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