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Invitrogen
IL-5 Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
IL-5 Antibody (500-P55BT-25UG) in ELISA
Product Details
500-P55BT-25UG
Species Reactivity
Host/Isotype
Class
Type
Immunogen
Conjugate
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Purification
Storage buffer
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RRID
Product Specific Information
AA Sequence of recombinant protein: MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-5. Anti-Murine IL-5-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Murine IL-5 by sandwich ELISA (using 100 mL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti- Murine IL-5 (500-P55) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-5.
Western Blot: To detect Murine IL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 mg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Murine IL-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P55BT-1MG will be provided as 2 x 500 µg
Target Information
IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Protein Aliases: B-cell growth factor II; BCGF-II; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; IL-5; ILN; Interleukin; Interleukin-5; Interleukin5; T-cell replacing factor; TRF
Gene Aliases: Il-5; Il5
UniProt ID: (Mouse) P04401
Entrez Gene ID: (Mouse) 16191
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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