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Invitrogen
IL-3 Polyclonal Antibody, Biotin, PeproTech®
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IL-3 Antibody (500-P53BT-25UG) in ELISA
Product Details
500-P53BT-25UG
Species Reactivity
Host/Isotype
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Type
Immunogen
Conjugate
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Product Specific Information
AA Sequence of recombinant protein: MDTHRLTRTL NCSSIVKEII GKLPEPELKT DDEGPSLRNK SFRRVNLSKF VESQGEVDPE DRYVIKSNLQ KLNCCLPTSA NDSALPGVFI RDLDDFRKKL RFYMVHLNDL ETVLTSRPPQ PASGSVSPNR GTVEC.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-3. Anti-Murine IL-3-specific antibody was purified by affinity chromatography and then biotinylated. Lyophilized from sterile-filtered PBS solution.
Sandwich ELISA: To detect mIL-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-3 (500-P53) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mIL-3.
Western Blot: To detect mIL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mIL-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P53BT-1MG will be provided as 2 x 500 µg
Target Information
Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. For example, human IL-3 has no activity on mouse cells. IL-3 is a potent growth promoting cytokine. IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types, and is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Protein Aliases: BP/BPA; Colony stimulating factor multiple; Eo-CSF; Hematopoietic growth factor; IL-3; ILN; Interleukin; Interleukin 3 (colony stimulating factor; Interleukin-3; Interleukin3; Mast cell growth factor; MCGF; MGC79398; MGC79399; MGF; Multi CSF; Multilineage colony stimulating factor; multiple); Multipotential colony-stimulating factor; P cell stimulating factor; P-cell-stimulating factor; PCSA
Gene Aliases: BPA; Csfmu; HCGF; Il-3; Il3; MCGF; PSF
UniProt ID: (Mouse) P01586
Entrez Gene ID: (Mouse) 16187
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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