Invitrogen

IKK beta Polyclonal Antibody

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Datasheet
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Datasheet
Protocols
Questions & Answers
IKK beta Polyclonal Antibody

Product Details

PA5-79456

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL
-

Immunocytochemistry (ICC/IF)

2 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human IKK beta recombinant protein (Position: E398-S756).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2746572

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: HEPG2 whole cell, COLO320 whole cell, M231 whole cell, HT1080 whole cell. IHC: Human Lung Cancer Tissue. ICC/IF: A431 cell. Flow: 293T cell.

Target Information

Phosphorylation of serine residues on the I-kappa-B proteins by IKK kinases, including IKBKB, marks them for destruction via the ubiquitination pathway, allowing activation of the NF-kappa-B complex. Inhibitor of nuclear factor kappa-B kinase subunit beta (IKK beta) is a serine kinase that plays an essential role in the NF-kaapa-B signaling pathway which is activated by mulitple stimuli such as inflammatory cytokines, bacterial, or viral products, DNA damages, or other cellular stresses. IKK beta acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Mutations affecting the IKK beta gene can result in Immunodeficiency 15 (IMD15).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ40509; I-kappa-B kinase 2; I-kappa-B-kinase beta; IKK-2; IKK-B; Inhibitor of nuclear factor kappa-B kinase subunit beta; MGC131801; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase beta; Serine/threonine protein kinase IKBKB

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Gene Aliases: IKBKB; IKK-beta; IKK2; IKKB; IMD15; NFKBIKB

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UniProt ID: (Human) O14920

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Entrez Gene ID: (Human) 3551

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Function(s)
protein kinase activity protein serine/threonine kinase activity protein binding ATP binding IkappaB kinase activity protein kinase binding protein homodimerization activity protein heterodimerization activity scaffold protein binding
Process(es)
stimulatory C-type lectin receptor signaling pathway skeletal muscle contraction protein phosphorylation inflammatory response I-kappaB kinase/NF-kappaB signaling I-kappaB phosphorylation positive regulation of cell proliferation response to virus response to toxic substance regulation of tumor necrosis factor-mediated signaling pathway positive regulation of neuron projection development cortical actin cytoskeleton organization neuron projection development response to lipopolysaccharide tumor necrosis factor-mediated signaling pathway negative regulation of myosin-light-chain-phosphatase activity TRIF-dependent toll-like receptor signaling pathway Fc-epsilon receptor signaling pathway regulation of phosphorylation response to drug serine phosphorylation of STAT protein negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling innate immune response positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity stress-activated MAPK cascade nucleotide-binding oligomerization domain containing signaling pathway interleukin-1-mediated signaling pathway response to fatty acid cellular response to tumor necrosis factor establishment of protein localization to plasma membrane positive regulation of neuron death regulation of establishment of endothelial barrier negative regulation of bicellular tight junction assembly
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