Invitrogen

Human IgM Polyclonal Antibody

Name: Human IgM Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-121216

View all (72) Human IgM antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

FIGURE: 1 / 1

Human IgM Antibody (PA5-121216) in WB

Western blot analysis of Human IgM in extracts of Raji cells. Samples were incubated with Human IgM Polyclonal antibody (Product # PA5-121216) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

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Immunogen image

Immunogen Image Human IgM Polyclonal Antibody

Product Details

PA5-121216

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

ELISA (ELISA)

1 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 213-453 of human IGHM (P01871)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.55 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2914788

Product Specific Information

Positive test controls include: Raji. The target is usually found in the following locations: Cell membrane, Secreted, Single-pass type I membrane protein.

Immunogen sequence: MCVPDQDTAI RVFAIPPSFA SIFLTKSTKL TCLVTDLTTY DSVTISWTRQ NGEAVKTHTN ISESHPNATF SAVGEASICE DDWNSGERFT CTVTHTDLPS PLKQTISRPK GVALHRPDVY LLPPAREQLN LRESATITCL VTGFSPADVF VQWMQRGQPL SPEKYVTSAP MPEPQAPGRY FAHSILTVSE EEWNTGETYT CVVAHEALPN RVTERTVDKS TGKPTLYNVS LVMSDTAGTC Y

Target Information

IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene Aliases: AGM1; MU; VH

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Entrez Gene ID: (Human) 3507