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  • Human IgA Antibodies

Invitrogen

Human IgA Monoclonal Antibody (3-F5)

View all (15) Human IgA antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Human IgA Monoclonal Antibody (3-F5)

  • Antibody Testing Data (1)
Human IgA Antibody in Western Blot (WB)
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Human IgA Antibody in Western Blot (WB)
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Human IgA Antibody (MA5-50628) in WB

Western blot analysis of Human IgA in human serum. Lysates/proteins at 10 µg/Lane. Predicted band size: 37 kDa. Observed band size: 60 kDa. Exposure time: 2 minutes; 8% SDS-PAGE gel. Primary antibody Human IgA monoclonal antibody (Product # MA5-50628) with a dilution of 1:500 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG-HRP Secondary Antibody with a dilution of 1:150,000 was used for 1 hour at room temperature. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room tem... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Human IgA Antibody in Western Blot (WB)
Human IgA Monoclonal Antibody (3-F5)

Product Details

MA5-50628

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1:1,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

3-F5

Immunogen

Native protein.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3093494

Product Specific Information

Positive control: Human serum.

Predicted band size: 37 kDa

Subcellular Location: Cell membrane, Immunoglobulin, Membrane, Secreted.

Target Information

Human IgA, also known as human immunoglobulin A, is an antibody that is produced by the immune system in response to antigens. It is the most abundant immunoglobulin in mucosal secretions, such as saliva, tears, and breast milk, and plays a key role in defense against pathogens at mucosal surfaces. IgA works by binding to specific antigens and preventing them from entering the body's tissues. It can also activate complement and promote phagocytosis by immune cells. Human IgA can be used in research to study the immune response to pathogens and to develop diagnostic tests and therapeutic interventions for infectious diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: A2m Marker; Ig alpha 1 Chain C Region; Ig alpha 2 Chain C Region; Immunoglobulin A; Immunoglobulin Am1; Immunoglobulin Am2; Immunoglobulin Heavy Constant Alpha 2

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Gene Aliases: IgA1

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Entrez Gene ID: (Human) 3493, (Human) 3494

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It has to be done as per old AB suggested Products section.
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