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  • Histone H4 Antibodies

Invitrogen

Histone H4 Recombinant Rabbit Monoclonal Antibody (SD209-04)

View all (57) Histone H4 antibodies

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Datasheet
Protocols
Questions & Answers

Cite Histone H4 Recombinant Rabbit Monoclonal Antibody (SD209-04)

  • Antibody Testing Data (11)
Histone H4 Antibody in Immunocytochemistry (ICC/IF)
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Histone H4 Antibody in Immunocytochemistry (ICC/IF)
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FIGURE: 1 / 11

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Histone H4 Antibody (MA5-32370) in ICC/IF

Immunocytochemical analysis of Histone H4 in PANC-1 cells using a Histone H4 Monoclonal antibody (Product # MA5-32370) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Histone H4 Antibody in Immunocytochemistry (ICC/IF)
Histone H4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Histone H4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Histone H4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Histone H4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Histone H4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Histone H4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Histone H4 Antibody in Western Blot (WB)
Histone H4 Antibody in Western Blot (WB)
Histone H4 Antibody in Western Blot (WB)
Histone H4 Antibody in Western Blot (WB)
Histone H4 Recombinant Rabbit Monoclonal Antibody (SD209-04)

Product Details

MA5-32370

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:20,000
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ChIP assay (ChIP)

0.5-2 µg
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SD209-04

Immunogen

Synthetic peptide within C-terminal Human Histone H4 aa 54-103
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2809651

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: H4 histone family, member A; H4 histone family, member E; H4 histone family, member I; H4 histone family, member J; H4 histone family, member M; H4 histone family, member N; H4 histone, family 2; histone 1, H4a; histone 1, H4b; histone 1, H4e; histone 1, H4i; histone 1, H4j; histone 2, H4; histone 2, H4a; Histone 4; Histone 4 family, member M; histone 4, H4; histone cluster 1, H4a; Histone Cluster 4; histone family member; Histone H4; Histone H4A; histone IV, family 2

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Gene Aliases: dJ160A22.2; FO108; H4; H4-12; H4-16; H4-53; H4/A; H4/B; H4/C; H4/D; H4/E; H4/G; H4/H; H4/I; H4/J; H4/K; H4/M; H4/N; H4/O; H4/p; H4C1; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4f16; H4F2; H4F2iv; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FM; H4FN; H4FO; H4M; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; Hist1h4m; HIST2H4; HIST2H4A; HIST2H4B; HIST4H4; X04652

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UniProt ID: (Human) P62805, (Mouse) P62806

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Entrez Gene ID: (Human) 8359, (Human) 8366, (Human) 8367, (Human) 8294, (Human) 8363, (Human) 8370, (Human) 121504, (Mouse) 326619, (Rat) 679983, (Mouse) 326620, (Mouse) 319158, (Mouse) 97122

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Function(s)
DNA binding protein binding protein domain specific binding histone binding poly(A) RNA binding protein heterodimerization activity
Process(es)
chromatin silencing at rDNA double-strand break repair via nonhomologous end joining nucleosome assembly DNA replication-dependent nucleosome assembly DNA replication-independent nucleosome assembly DNA-templated transcription, initiation telomere capping gene silencing by RNA telomere organization CENP-A containing nucleosome assembly cellular protein metabolic process negative regulation of megakaryocyte differentiation negative regulation of gene expression, epigenetic positive regulation of gene expression, epigenetic protein heterotetramerization beta-catenin-TCF complex assembly
It has to be done as per old AB suggested Products section.
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