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Histone H3 Monoclonal Antibody (1A2A3)

View all (191) Histone H3 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Histone H3 Monoclonal Antibody (1A2A3)

  • Antibody Testing Data (11)
Histone H3 Antibody in Immunocytochemistry (ICC/IF)
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Histone H3 Antibody in Immunocytochemistry (ICC/IF)
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FIGURE: 1 / 11

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Histone H3 Antibody (68345-1-IG) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Histone H3 antibody (68345-1-Ig, Clone: 1A2A3, labeled with CoraLite647-conjugated AffiniPure F(ab)2 Fragment Goat Anti-Mouse IgG (H+L),SA00014-10, magenta) at dilution of 1:400 and CoraLite Plus 488-conjugated ZO-1 antibody (CL488-21773, green), CHCHD6 antibody (66597-1-Ig, Clone: 2A11E9, labeled with FlexAble CoraLite Plus 555 Antibody Labelin... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Histone H3 Antibody in Immunocytochemistry (ICC/IF)
Histone H3 Antibody in Immunocytochemistry (ICC/IF)
Histone H3 Antibody in Immunocytochemistry (ICC/IF)
Histone H3 Antibody in Immunocytochemistry (ICC/IF)
Histone H3 Antibody in Immunocytochemistry (ICC/IF)
Histone H3 Antibody in Immunocytochemistry (ICC/IF)
Histone H3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Histone H3 Antibody in Western Blot (WB)
Histone H3 Antibody in Western Blot (WB)
Histone H3 Antibody in Flow Cytometry (Flow)
Histone H3 Antibody in Immunoprecipitation (IP)

Product Details

68345-1-IG

Applications
Tested Dilution
Publications

Western Blot (WB)

1:5,000-1:50,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000
-

Immunocytochemistry (ICC/IF)

1:500-1:2,000
-

Flow Cytometry (Flow)

0.4 µg/1x10^6 cell suspension
-

ELISA (ELISA)

Assay-dependent
-

Immunoprecipitation (IP)

0.5-4 µg/1-3 mg of protein lysate
-
Product Specifications

Species Reactivity

Dog, Chicken, Hamster, Human, Mouse, Plant, Pig, Rabbit, Rat, Zebrafish

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

1A2A3

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated
  • CoraLite Plus 488
  • Request custom conjugation

Form

Liquid

Purification

Protein A

Storage buffer

PBS with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: H3 histone family, member A; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3-clustered histone 2; H3-clustered histone 3; H3-clustered histone 4; H3-clustered histone 6; H3-clustered histone 7; H3/b; H3/d; H3/f; H3/j; H3S10ph; histone 1, H2ai; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 1, H3j; histone 2, H3a; histone 2, H3c; histone 2, H3c2; histone 2, H3ca2; Histone 3; histone cluster 1, H2ai; histone cluster 1, H2ai-like; histone cluster 1, H3a; Histone Cluster 2 H3a; histone cluster 2, H3c2; histone cluster 2, H3c2, pseudogene; histone cluster 3, H3; histone gene complex 1; histone H3; Histone H3.1; Histone H3.2; Histone H3.3; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o; uncharacterized LOC100856216

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Gene Aliases: H3; H3 histone; H3-143; H3-291; h3-5; H3-53; H3-614; H3-B; H3-F; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; H3/A; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3a; H3b; H3C1; H3C10; H3C11; H3C12; H3C13; H3C14; H3C15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3f; H3F1K; H3F2; h3f3a; h3f3b.1; h3f3c; h3f3d; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; H3g; H3h; H3i; Hist1; Hist1h2ai; Hist1h2ail; HIST1H3A; HIST1H3B; Hist1h3c; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1; Hist2h3c2; Hist2h3c2-ps; Hist2h3ca1; Hist2h3ca2; HIST2H3D; M32461; wu:fa25h06; wu:fa96g06; wu:fb07a08; wu:fb36f01; zgc:110292; zgc:174300; zgc:56193; zgc:56418; zgc:64222; zgc:86731

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UniProt ID: (Human) P68431, (Human) Q71DI3, (Rat) Q6LED0, (Mouse) P68433, (Mouse) P84228, (Zebrafish) Q6PI20

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Entrez Gene ID: (Human) 8350, (Human) 8358, (Human) 8968, (Human) 8357, (Human) 8356, (Human) 333932, (Human) 126961, (Rat) 291159, (Mouse) 360198, (Rat) 679994, (Mouse) 319150, (Mouse) 260423, (Mouse) 319152, (Mouse) 97114, (Rat) 310678, (Dog) 100856216, (Zebrafish) 550262

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Function(s)
DNA binding protein binding histone binding protein heterodimerization activity cadherin binding involved in cell-cell adhesion chromatin binding
Process(es)
positive regulation of defense response to virus by host nucleosome assembly DNA replication-dependent nucleosome assembly protein heterotetramerization regulation of gene silencing chromatin silencing at rDNA blood coagulation gene silencing by RNA cellular protein metabolic process negative regulation of gene expression, epigenetic positive regulation of gene expression, epigenetic telomere organization cell-cell adhesion xenophagy negative regulation of transcription from RNA polymerase II promoter
It has to be done as per old AB suggested Products section.
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