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  • Primary Antibodies ›
  • HMGCS2 Antibodies

Invitrogen

HMGCS2 Polyclonal Antibody

View all (11) HMGCS2 antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Cite HMGCS2 Polyclonal Antibody

  • Antibody Testing Data (4)
HMGCS2 Antibody in Immunocytochemistry (ICC/IF)
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HMGCS2 Antibody in Immunocytochemistry (ICC/IF)
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HMGCS2 Antibody (PA5-112573) in ICC/IF

Immunocytochemichal analysis of HMGCS2 in HepG2 cells using a Polyclonal antibody (Product # PA5-112573) at a dilution of 1:133. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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HMGCS2 Antibody in Immunocytochemistry (ICC/IF)
HMGCS2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
HMGCS2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
HMGCS2 Antibody in Western Blot (WB)
HMGCS2 Polyclonal Antibody

Product Details

PA5-112573

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:5,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human Hydroxymethylglutaryl-CoA synthase, mitochondrial protein (426-508AA)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.79 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.03% ProClin 300

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2867308

Target Information

The protein encoded by this gene belongs to the HMG-CoA synthase family. It is a mitochondrial enzyme that catalyzes the first reaction of ketogenesis, a metabolic pathway that provides lipid-derived energy for various organs during times of carbohydrate deprivation, such as fasting. Mutations in this gene are associated with HMG-CoA synthase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 3-hydroxy-3-methylglutary-Coenzyme A synthase 2; 3-hydroxy-3-methylglutaryl coenzyme A synthase; 3-hydroxy-3-methylglutaryl-CoA synthase 2 (mitochondrial); 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial); HMG-CoA synthase; hydroxymethylglutaryl-CoA synthase 2; Hydroxymethylglutaryl-CoA synthase, mitochondrial; testicular tissue protein Li 88

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Gene Aliases: Hmgcs1; HMGCS2; Mt3h3mg

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UniProt ID: (Human) P54868, (Rat) P22791

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Entrez Gene ID: (Human) 3158, (Rat) 24450

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Function(s)
hydroxymethylglutaryl-CoA synthase activity
Process(es)
kidney development liver development cholesterol biosynthetic process brain development midgut development response to nutrient isoprenoid biosynthetic process response to temperature stimulus response to bacterium response to metal ion response to organic cyclic compound lung development response to insulin cellular response to insulin stimulus cellular response to hormone stimulus multicellular organismal response to stress response to testosterone response to glucagon response to triglyceride response to monosaccharide response to prostaglandin F response to drug response to starvation response to peptide hormone response to ethanol ketone body biosynthetic process response to glucocorticoid response to cAMP response to growth hormone adipose tissue development response to fatty acid response to linoleic acid cellular response to lipopolysaccharide cellular response to amino acid stimulus cellular response to glucocorticoid stimulus cellular response to fatty acid cellular response to organic cyclic compound
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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