Invitrogen

HMGB2 Recombinant Rabbit Monoclonal Antibody (8P10V5)

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Datasheet
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Datasheet
Protocols
Questions & Answers
HMGB2 Recombinant Rabbit Monoclonal Antibody (8P10V5)

Product Details

MA5-35771

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:6,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:2,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-

ELISA (ELISA)

1 µg/mL
-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of
-

ChIP assay (ChIP)

5 µg antibody for 10 µg-15 µg of Chromatin
-

CUT&RUN (C&R)

105 cells /1 µg
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

8P10V5

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HMGB2 (P26583)
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.14 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849671

Product Specific Information

Immunogen sequence: MGKGDPNKPR GKMSSYAFFV QTCREEHKKK HPDSSVNFAE FSKKCSERWK TMSAKEKSKF EDMAKSDKAR YDREMKNYVP PKGDKKGKKK DPNAPKRPPS

Target Information

HMGB2 encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: High mobility group protein 2; High mobility group protein B2; high-mobility group (nonhistone chromosomal) protein 2; high-mobility group box 2; HMG 2; HMG B2; HMG-2; HMG-B2; HMGB-2

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Gene Aliases: C80539; HMG-2; HMG2; HMGB2

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UniProt ID: (Human) P26583, (Rat) P52925, (Mouse) P30681

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Entrez Gene ID: (Human) 3148, (Rat) 29395, (Mouse) 97165

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Function(s)
four-way junction DNA binding enhancer sequence-specific DNA binding DNA binding damaged DNA binding double-stranded DNA binding single-stranded DNA binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding drug binding DNA binding, bending protein domain specific binding chemoattractant activity transcription regulatory region DNA binding non-sequence-specific DNA binding, bending poly(A) RNA binding RAGE receptor binding supercoiled DNA binding lipid binding nucleosomal histone binding chromatin binding
Process(es)
positive regulation of endothelial cell proliferation base-excision repair, DNA ligation chromatin remodeling regulation of transcription from RNA polymerase II promoter spermatogenesis spermatid nucleus differentiation nervous system development male gonad development organ regeneration positive regulation of nuclease activity positive regulation of DNA binding positive regulation of erythrocyte differentiation positive regulation of megakaryocyte differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter response to steroid hormone positive chemotaxis positive regulation of DNA metabolic process cell chemotaxis cellular response to lipopolysaccharide negative regulation of extrinsic apoptotic signaling pathway via death domain receptors inflammatory response to antigenic stimulus DNA topological change apoptotic DNA fragmentation chromatin organization nucleosome assembly transcription, DNA-templated DNA geometric change response to lipopolysaccharide positive regulation of interferon-beta production V(D)J recombination response to drug innate immune response positive regulation of innate immune response regulation of neurogenesis defense response to Gram-negative bacterium defense response to Gram-positive bacterium DNA ligation involved in DNA repair regulation of stem cell proliferation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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