Osenses

HECT domain of NEDD4 Polyclonal Antibody

2 References
View all (34) NEDD4 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

OSN00011W-100UL

Applications
Tested Dilution
Publications

Western Blot (WB)

1:300-1:2,000
-

Immunohistochemistry (IHC)

1:300-1:2,000
-

Miscellaneous PubMed (MISC)

-
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / Ig

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide from the HECT domain of human NEDD4 conjugated to an immunogenic carrier protein was used as the antigen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.

The peptide is homologous in rat and mouse.

Specificity of this antibody: NEDD4.

Target Information

The E3 ubiquitin-protein ligase NEDD4 is a Ubl conjugation pathway protein. It contains one HECT (E6AP-type E3 ubiquitin-protein ligase) domain and four WW domains. It is involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. It acts as a potential proto-oncogene that negatively regulates PTEN via ubiquitination, a paradigm analogous to that of Mdm2 and p53. It is also reported to be involved in the budding of many viruses and is the regulator of epithelial Na+ channels, which lead to arterial hypertension. NEDD4 protein is localized to the sarcolemmal region of muscle fibers. Increase in NEDD4 protein expression leads to unloaded and denervated muscles as well as a decrease in Notch1 expression. Hence it acts as down regulator of Notch-1 gene expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Cell proliferation-inducing gene 53 protein; E3 ubiquitin-protein ligase NEDD4; HECT-type E3 ubiquitin transferase NEDD4; NEDD-4; Neural precursor cell expressed developmentally down-regulated protein 4; neural precursor cell expressed, developmentally down-regulated gene 4; neural precursor cell expressed, developmentally down-regulated gene 4A; neural precursor cell expressed, developmentally down-regulted gene 4; OTTHUMP00000217840; OTTHUMP00000217841; OTTHUMP00000217842; receptor-potentiating factor 1

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Gene Aliases: AA959633; AL023035; AU019897; E430025J12Rik; KIAA0093; mKIAA0093; Nedd-4; NEDD4; NEDD4-1; Nedd4a; PIG53; RPF1

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UniProt ID: (Human) P46934, (Rat) Q62940, (Mouse) P46935

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Entrez Gene ID: (Human) 4734, (Rat) 25489, (Mouse) 17999

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Function(s)
protein binding protein C-terminus binding ligase activity sodium channel inhibitor activity protein domain specific binding beta-2 adrenergic receptor binding ubiquitin binding phosphoserine binding phosphothreonine binding ubiquitin protein ligase activity RNA polymerase binding proline-rich region binding ubiquitin-protein transferase activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter adaptive immune response outflow tract morphogenesis endocardial cushion development protein monoubiquitination protein targeting to lysosome immune response lysosomal transport neuromuscular junction development negative regulation of sodium ion transport negative regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage positive regulation of phosphatidylinositol 3-kinase signaling response to denervation involved in regulation of muscle adaptation protein ubiquitination transmission of virus negative regulation of vascular endothelial growth factor receptor signaling pathway neuron projection development receptor internalization receptor catabolic process cellular response to UV regulation of ion transmembrane transport T cell activation regulation of membrane potential protein ubiquitination involved in ubiquitin-dependent protein catabolic process glucocorticoid receptor signaling pathway ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway development involved in symbiotic interaction positive regulation of protein catabolic process positive regulation of nucleocytoplasmic transport blood vessel morphogenesis regulation of dendrite morphogenesis regulation of synapse organization progesterone receptor signaling pathway protein K63-linked ubiquitination regulation of potassium ion transmembrane transporter activity regulation of macroautophagy response to calcium ion negative regulation of sodium ion transmembrane transporter activity nervous system development
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