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Invitrogen
HBEGF Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 1
HBEGF Antibody (500-P329BT-25UG) in ELISA
Product Details
500-P329BT-25UG
Species Reactivity
Host/Isotype
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Type
Immunogen
Conjugate
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Product Specific Information
AA Sequence of recombinant protein: DLQEADLDLL RVTLSSKPQA LATPNKEEHG KRKKKGKGLG KKRDPCLRKY KDFCIHGECK YVKELRAPSC ICHPGYHGER CHGLSL.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human HB-EGF. Anti-Human HB-EGF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human HB-EGF by sandwich ELISA (using 100 µL/well), a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human HB-EGF (500-P329) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human HB-EGF.
Western Blot: To detect Human HB-EGF by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human HB-EGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P329BT-1MG will be provided as 2 x 500 µg
Target Information
HBEGF is a growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. It is required for normal cardiac valve formation and normal heart function. HBEGF promotes smooth muscle cell proliferation. It may be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. It also acts as a diphtheria toxin receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Protein Aliases: diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor); hb egf; heparin-binding EGF-like growth factor; heparin-binding epidermal growth factor; Proheparin-binding EGF-like growth factor
Gene Aliases: DTR; DTS; DTSF; HBEGF; HEGFL
UniProt ID: (Human) Q99075
Entrez Gene ID: (Human) 1839
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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