Invitrogen

GRASP65 Monoclonal Antibody (OTI5G8)

Name: GRASP65 Monoclonal Antibody (OTI5G8)
Brand: Invitrogen
SKU: MA5-25148

1 Reference

View all (18) GRASP65 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (8)

GRASP65 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

GRASP65 Antibody (MA5-25148) in ICC/IF

Immunofluorescence analysis of Golgi reassembly-stacking protein 1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with GRASP65 Monoclonal Antibody (OTI5G8) (Product # MA5-25148) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibo... View More

GRASP65 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Immunogen image

Immunogen Image GRASP65 Monoclonal Antibody (OTI5G8)

Product Details

MA5-25148

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50

Immunocytochemistry (ICC/IF)

1:100

Flow Cytometry (Flow)

1:100

Product Specifications

Species Reactivity

Dog, Human, Non-human primate, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI5G8

Immunogen

Full length human recombinant protein of GORASP1 produced in HEK293T cell

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2724399

Target Information

Grasp65 is a structural protein of the golgi apparatus. It has been implicated in the stacking of golgi cisternae. Grasp65 is shown to have the properties to bind surfaces together in a mitotically regulated matter.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Golgi peripheral membrane protein p65; Golgi phosphoprotein 5; Golgi reassembly and stacking protein 1; golgi reassembly stacking protein 1, 65kDa; Golgi reassembly-stacking protein 1; Golgi reassembly-stacking protein of 65 kDa; GOLPH5; GRASP65

View more View less

Gene Aliases: GOLPH5; GORASP1; GRASP65; P65

View more View less

UniProt ID: (Human) Q9BQQ3

View more View less

Entrez Gene ID: (Human) 64689, (Dog) 485594, (Rat) 56082

View more View less

Function(s)

protein binding

Process(es)

Golgi organization protein transport negative regulation of dendrite morphogenesis protein N-linked glycosylation ER to Golgi vesicle-mediated transport COPII vesicle coating

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

GRASP65 Monoclonal Antibody (OTI5G8)

GRASP65 Antibody (MA5-25148) in ICC/IF

Product Details

MA5-25148

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!