Disclaimer

Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

  • Primary Antibodies ›
  • GOT1 Antibodies

Invitrogen

GOT1 Monoclonal Antibody (GT1255)

View all (31) GOT1 antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite GOT1 Monoclonal Antibody (GT1255)

  • Antibody Testing Data (5)
GOT1 Antibody in Immunocytochemistry (ICC/IF)
Group 53 Created with Sketch.
GOT1 Antibody in Immunocytochemistry (ICC/IF)
Group 53 Created with Sketch.

FIGURE: 1 / 5

{{ $ctrl.currentElement.advancedVerification.fullName }}
{{ $ctrl.currentElement.advancedVerification.text }}

GOT1 Antibody (MA5-27751) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of GOT1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GOT1 Monoclonal Antibody (GT1255) (Product # MA5-27751) diluted at 1:100. Blue: Hoechst 33342 staining. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
Published figure supplied by benchsci-logo
PMID: {{$ctrl.currentElement.benchSciPubmedId}}
View Product

{{$ctrl.videoDesc}}

{{$ctrl.videoLongDesc}}

GOT1 Antibody in Immunocytochemistry (ICC/IF)
GOT1 Antibody in Western Blot (WB)
GOT1 Antibody in Western Blot (WB)
GOT1 Antibody in Western Blot (WB)
GOT1 Antibody in Western Blot (WB)
GOT1 Monoclonal Antibody (GT1255)

Product Details

MA5-27751

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:10,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

GT1255

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human GOT1. The exact sequence is proprietary.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2735206

Product Specific Information

Positive Control: 293T, A431, HeLa, HepG2, mouse heart, mouse liver

Predicted Reactivity: Rat (87%), Zebrafish (81%), Dog (100%), Pig (97%), Chicken (81%), Chimpanzee (100%), Bovine (97%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

GOT1 is a glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: aspartate aminotransferase 1; Aspartate aminotransferase, cytoplasmic; aspartate transaminase 1; cAspAT; cCAT; Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cytosolic aspartate aminotransferase; Glutamate oxaloacetate transaminase 1; glutamate oxaloacetate transaminase 1, soluble; glutamic-oxaloacetic transaminase 1, soluble; growth-inhibiting protein 18; testis secretory sperm-binding protein Li 196a; Transaminase A

View more View less

Gene Aliases: AI789014; AST1; ASTQTL1; cAspAT; cCAT; GIG18; Got-1; GOT1

View more View less

UniProt ID: (Human) P17174, (Mouse) P05201

View more View less

Entrez Gene ID: (Human) 2805, (Mouse) 14718

View more View less

Function(s)
L-aspartate:2-oxoglutarate aminotransferase activity phosphatidylserine decarboxylase activity pyridoxal phosphate binding carboxylic acid binding identical protein binding L-cysteine:2-oxoglutarate aminotransferase activity L-phenylalanine:2-oxoglutarate aminotransferase activity catalytic activity transaminase activity transferase activity
Process(es)
oxaloacetate metabolic process glycerol biosynthetic process cellular amino acid metabolic process aspartate metabolic process aspartate biosynthetic process aspartate catabolic process Notch signaling pathway cellular amino acid biosynthetic process biosynthetic process glutamate catabolic process to aspartate glutamate catabolic process to 2-oxoglutarate dicarboxylic acid metabolic process fatty acid homeostasis gluconeogenesis 2-oxoglutarate metabolic process glutamate metabolic process cellular response to insulin stimulus response to glucocorticoid
It has to be done as per old AB suggested Products section.
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support

Your items have has been added!


Host server : magellan-search-c7d89468c-sbmwt:80/100.66.128.142:80.
git-commit: 4db89d68e0ee702f860d53b64def1eb94897fade
git-url: https://github.com/thermofisher/magellan-search
git-branch: release/2.36.2-2025.09.39-1.0